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Titolo:
QUAIL EGG-YOLK - A NOVEL CRYOPROTECTANT FOR THE FREEZE PRESERVATION OF POITOU JACKASS SPERM
Autore:
TRIMECHE A; ANTON M; RENARD P; GANDEMER G; TAINTURIER D;
Indirizzi:
ECOLE NATL VET,LAB SCI CLIN & PATHOL REPROD,BP 40706 F-44307 NANTES 03 FRANCE INRA,LEIMA,GRP LIPIDES FLAVEUR F-44316 NANTES 03 FRANCE CHR HOTEL DIEU,GRP RECH APPL & FERTILITE F-35000 RENNES FRANCE
Titolo Testata:
Cryobiology
fascicolo: 4, volume: 34, anno: 1997,
pagine: 385 - 393
SICI:
0011-2240(1997)34:4<385:QE-ANC>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
BOVINE SPERMATOZOA; MOTILITY; PHOSPHOLIPIDS; CHOLESTEROL; SEPARATION; PROTEINS; LIPIDS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
A. Trimeche et al., "QUAIL EGG-YOLK - A NOVEL CRYOPROTECTANT FOR THE FREEZE PRESERVATION OF POITOU JACKASS SPERM", Cryobiology, 34(4), 1997, pp. 385-393

Abstract

For many years, attempts have been made to establish a sperm bank forthe Poitou jackass population which is threatened with extinction. Unfortunately, no cryopreservation technique has ever been described forspermatozoa of this species. In an attempt to find a suitable technique, we studied the relative effectiveness of chicken egg yolk and quail egg yolk in preserving the motility and characteristics of movement of Poitou jackass spermatozoa during the freezing-thawing process. Semen was diluted to 60 x 10(6) sperm/ml in a preservation medium containing 4% (v/v) glycerol with 0, 2, 5, 10, 15, or 20% (v/v) of chicken orquail egg yolk. The chemical composition of these two eggs was compared. Effects were assessed using an automated analyzer which measured curvilinear velocity (VCL), straight line velocity (VSL), and the velocity of the average path. Linearity was defined as VSL/VCL x 100. The amplitude of the lateral head displacement was also measured. It was found that after the freeze-thaw process, quail egg yolk improved the percentages of motile and progressively undulating spermatozoa and the movement characteristics compared with chicken egg yolk. The optimal concentration of quail egg yolk was 10%. The general composition of the two types of egg yolk were similar, but quail egg yolk contained significantly more phosphatidylcholine, less phosphatidylethanolamine, and a smaller ratio of polyunsaturated to saturated fatty acids than chicken egg yolk. The improvement of motility for frozen-thawed Poitou jackass spermatozoa using frozen-thawed quail egg yolk compared to chickenegg yolk may be due to the differences in composition of the two yolks. (C) 1997 Academic Press.

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Documento generato il 02/12/20 alle ore 15:18:23