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Titolo:
The vectorial competence of Phlebotomus sergenti is specific for Leishmania tropica and is controlled by species-specific, lipophosphoglycan-mediatedmidgut attachment
Autore:
Kamhawi, S; Modi, GB; Pimenta, PFP; Rowton, E; Sacks, DL;
Indirizzi:
NIAID, Parasit Dis Lab, NIH, Bethesda, MD 20892 USA NIAID Bethesda MD USA20892 Parasit Dis Lab, NIH, Bethesda, MD 20892 USA Fiocruz MS, Ctr Pesquisas Rene Rachou, Lab Entomol Med, BR-30190002 Belo Horizonte, MG, Brazil Fiocruz MS Belo Horizonte MG Brazil BR-30190002 BCo Horizonte, MG, Brazil Walter Reed Army Med Ctr, Dept Entomol, Washington, DC 20307 USA Walter Reed Army Med Ctr Washington DC USA 20307 Washington, DC 20307 USA
Titolo Testata:
PARASITOLOGY
, volume: 121, anno: 2000,
parte:, 1
pagine: 25 - 33
SICI:
0031-1820(200007)121:<25:TVCOPS>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
SANDFLY VECTOR; DONOVANI LIPOPHOSPHOGLYCAN; SURFACE LIPOPHOSPHOGLYCAN; CUTANEOUS LEISHMANIASIS; PAPATASI; PROMASTIGOTES; IDENTIFICATION; POLYMORPHISMS; PURIFICATION; PSYCHODIDAE;
Keywords:
Phlebotomus sergenti; Leishmania tropica; lipophosphoglycan; vector specificity;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Sacks, DL NIAID, Parasit Dis Lab, NIH, Bldg 4,Room 126, Bethesda, MD 20892USA NIAID Bldg 4,Room 126 Bethesda MD USA 20892 thesda, MD 20892 USA
Citazione:
S. Kamhawi et al., "The vectorial competence of Phlebotomus sergenti is specific for Leishmania tropica and is controlled by species-specific, lipophosphoglycan-mediatedmidgut attachment", PARASITOL, 121, 2000, pp. 25-33

Abstract

The vectorial competence of Phlebotomus sergenti for 3 Old World species of Leishmania, L. tropica, L. major and L. donovani, was investigated in vivo and by in vitro midgut binding assays using living promastigotes and purified lipophosphoglycan (LPG). P. sergenti consistently showed a high specificity for L. tropica strains, which were able to develop mature, potentially transmissible infections. The loss of infection with L. major and L. donovani correlated with the excretion of the digested bloodmeal. These strainswere able to produce sustained infections in the midguts of their appropriate vectors, P. papatasi and P. argentipes, respectively. In in vitro binding assays, a significantly higher number of L. tropica procyclic promastigotes attached to the midgut lining of P. sergenti, compared to those of L. major and L. donovani (P < 0.05). The prediction that the species specificity of midgut attachment is controlled by polymorphic structures on the parasite LPG was supported by the finding that P. sergenti midguts were intensely stained following incubation with purified phosphoglycan (PG) from L. tropica compared with PGs from L. major or L. donovani. The results provide further evidence that LPG structural polymorphisms are driven by the species diversity of molecules present on the sandfly midgut that function as parasite attachment sites.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/08/20 alle ore 19:49:51