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Titolo:
Molecular characterization of the murine orthologue of the human retinal proteoglycan IPM 150
Autore:
Kuehn, M; Wietecki, D; Hageman, G;
Indirizzi:
Univ Iowa, Dept Ophthalmol & Visual Sci, Ctr Macular Degenerat, Iowa City,IA 52240 USA Univ Iowa Iowa City IA USA 52240 acular Degenerat, Iowa City,IA 52240 USA
Titolo Testata:
MOLECULAR VISION
fascicolo: 18, volume: 6, anno: 2000,
pagine: 148 - 156
SICI:
1090-0535(20000824)6:18<148:MCOTMO>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERPHOTORECEPTOR MATRIX PROTEOGLYCANS; CELL-ADHESION; IMMUNOCYTOCHEMICAL LOCALIZATION; SULFATE PROTEOGLYCAN; PIGMENT-EPITHELIUM; VERTEBRATE RETINA; SEQUENCE-ANALYSIS; BINDING PROTEINS; DERMATAN SULFATE; RAT RETINA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
65
Recensione:
Indirizzi per estratti:
Indirizzo: Hageman, G Univ Iowa, Dept Ophthalmol & Visual Sci, Ctr Macular Degenerat,1190E PFP,200 Hawkins Dr, Iowa City, IA 52240 USA Univ Iowa 1190E PFP,200 Hawkins Dr Iowa City IA USA 52240 0 USA
Citazione:
M. Kuehn et al., "Molecular characterization of the murine orthologue of the human retinal proteoglycan IPM 150", MOL VIS, 6(18), 2000, pp. 148-156

Abstract

PURPOSE: We recently identified a family of novel human proteoglycans/glycoproteins that are major constituents of the human interphotoreceptor matrix. Two members of this family, designated IPM 150 and IPM 200, have been extensively characterized. Although the IPM is thought to mediate crucial roles in retinal physiology, including retinal adhesion and photoreceptor cellviability, little is known about the roles of specific IPM constituents inthese processes. In order to characterize the mouse IPM 150 orthologue, toinitiate functional in vivo studies, and as a prerequisite towards future genetic manipulation, we cloned the murine orthologue of human IPM 150 and determined its chromosomal location. METHODS: A mouse retinal cDNA library was screened using an IMAGE clone with sequence similarity to human IPM 150. The genomic location of the mouse IPM 150 gene was determined by radiation hybrid analyses. RESULTS: We describe here the molecular structure of the murine orthologueof human IPM 150 and place the location of its gene on mouse chromosome 9. Among the tissues examined, expression of IPM 150 appeared to be restricted to the retina. CONCLUSIONS: Comparison of the human and murine IPM 150 core proteins revealed that the molecules are generally well conserved, although several potentially significant differences do exist. In addition, two highly conserveddomains within the core proteins were identified. The data presented here represent a first step towards the development of experimental murine models, which may eventually be used to elucidate the mechanisms underlying retinal adhesion and photoreceptor survival.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 18:39:32