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Titolo:
Evidence for a second function for Saccharomyces cerevisiae Rev1p
Autore:
Nelson, JR; Gibbs, PEM; Nowicka, AM; Hinkle, DC; Lawrence, CW;
Indirizzi:
Univ Rochester, Sch Med & Dent, Dept Biochem & Biophys, Rochester, NY 14642 USA Univ Rochester Rochester NY USA 14642 & Biophys, Rochester, NY 14642 USA Univ Rochester, Coll Arts & Sci, Dept Biol, Rochester, NY 14642 USA Univ Rochester Rochester NY USA 14642 Dept Biol, Rochester, NY 14642 USA
Titolo Testata:
MOLECULAR MICROBIOLOGY
fascicolo: 3, volume: 37, anno: 2000,
pagine: 549 - 554
SICI:
0950-382X(200008)37:3<549:EFASFF>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI DINB; THYMINE CYCLOBUTANE DIMER; SINGLE-STRANDED VECTOR; YEAST DNA-POLYMERASE; RADIATION SENSITIVITY; GENE ENCODES; MUTAGENESIS; FREQUENCY; MUTATIONS; PHOTOPRODUCT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Lawrence, CW Univ Rochester, Sch Med & Dent, Dept Biochem & Biophys, Rochester, NY 14642 USA Univ Rochester Rochester NY USA 14642 ochester, NY 14642 USA
Citazione:
J.R. Nelson et al., "Evidence for a second function for Saccharomyces cerevisiae Rev1p", MOL MICROB, 37(3), 2000, pp. 549-554

Abstract

The function of the Saccharomyces cerevisiae REV1 gene is required for translesion replication and mutagenesis induced by a wide variety of DNA-damaging agents. We showed previously that Rev1p possesses a deoxycytidyl transferase activity, which incorporates dCMP opposite abasic sites in the DNA template, and that dCMP insertion is the major event during bypass of an abasic site in vivo. However, we now find that Rev1p function is needed for thebypass of a T-T (6-4) UV photoproduct, a process in which dCMP incorporation occurs only very rarely, indicating that Rev1p possesses a second function. In addition, we find that Rev1p function is, as expected, required for bypass of an abasic site. However, replication past this lesion was also much reduced in the G-193R rev1-1 mutant, which we find retains substantial levels of deoxycytidyl transferase activity. This mutant is, therefore, presumably deficient principally in the second, at present poorly defined, function. The bypass of an abasic site and T-T (6-4) lesion also depended on REV3 function, but neither it nor REV1 was required for replication past the T-T dimer; bypass of this lesion presumably depends on another enzyme.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 00:26:13