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Titolo:
EEA1, a tethering protein of the early sorting endosome, shows a polarizeddistribution in hippocampal neurons, epithelial cells, and fibroblasts
Autore:
Wilson, JM; de Hoop, M; Zorzi, N; Toh, BH; Dotti, CG; Parton, RG;
Indirizzi:
Univ Queensland, Dept Physiol & Pharmacol, Ctr Microscopy & Microanal, Brisbane, Qld 4072, Australia Univ Queensland Brisbane Qld Australia 4072 Brisbane, Qld 4072, Australia Univ Queensland, Ctr Cellular & Mol Biol, Brisbane, Qld 4072, Australia Univ Queensland Brisbane Qld Australia 4072 Brisbane, Qld 4072, Australia Univ Arizona, Dept Cell Biol & Anat, Tucson, AZ USA Univ Arizona Tucson AZ USA rizona, Dept Cell Biol & Anat, Tucson, AZ USA European Mol Biol Lab, Heidelberg, Germany European Mol Biol Lab Heidelberg Germany Biol Lab, Heidelberg, Germany Monash Univ, Dept Pathol & Immunol, Melbourne, Vic 3004, Australia Monash Univ Melbourne Vic Australia 3004 , Melbourne, Vic 3004, Australia
Titolo Testata:
MOLECULAR BIOLOGY OF THE CELL
fascicolo: 8, volume: 11, anno: 2000,
pagine: 2657 - 2671
SICI:
1059-1524(200008)11:8<2657:EATPOT>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
BASOLATERAL ENDOCYTIC PATHWAYS; SUCKLING RAT ILEUM; MDCK CELLS; TRANSFERRIN RECEPTORS; NONPOLARIZED CELLS; MEMBRANE-PROTEINS; CULTURED NEURONS; ABSORPTIVE CELLS; AUTOANTIGEN EEA1; PLASMA-MEMBRANE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Parton, RG Univ Queensland, Dept Physiol & Pharmacol, Ctr Microscopy & Microanal, Brisbane, Qld 4072, Australia Univ Queensland Brisbane Qld Australia 4072 ld 4072, Australia
Citazione:
J.M. Wilson et al., "EEA1, a tethering protein of the early sorting endosome, shows a polarizeddistribution in hippocampal neurons, epithelial cells, and fibroblasts", MOL BIOL CE, 11(8), 2000, pp. 2657-2671

Abstract

EEA1 is an early endosomal Rab5 effector protein that has been implicated in the docking of incoming endocytic vesicles before fusion with early endosomes. Because of the presence of complex endosomal pathways in polarized and nonpolarized cells, we have examined the distribution of EEA1 in diversecell types. Ultrastructural analysis demonstrates that EEA1 is present on a subdomain of the early sorting endosome but not on clathrin-coated vesicles, consistent with a role in providing directionality to early endosomal fusion. Furthermore, EEA1 is associated with filamentous material that extends from the cytoplasmic surface of the endosomal domain, which is also consistent with a tethering/docking role for EEA1. In polarized cells (Madin-Darby canine kidney cells and hippocampal neurons), EEA1 is present on a subset of "basolateral-type" endosomal compartments, suggesting that EEA1 regulates specific endocytic pathways. In both epithelial cells and fibroblasticcells, EEA1 and a transfected apical endosomal marker, endotubin, label distinct endosomal populations. Hence, there are at least two distinct sets of early endosomes in polarized and nonpolarized mammalian cells. EEA1 couldprovide specificity and directionality to fusion events occurring in a subset of these endosomes in polarized and nonpolarized cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 00:59:02