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Titolo:
Molecular cloning, distribution and pharmacological characterization of a novel gonadotropin-releasing hormone ([Trp(8)] GnRH) in frog brain
Autore:
Yoo, MS; Kang, HM; Choi, HS; Kim, JW; Troskie, BE; Millar, RP; Kwon, HB;
Indirizzi:
Chonnam Natl Univ, Hormone Res Ctr, Kwangju 500757, South Korea Chonnam Natl Univ Kwangju South Korea 500757 Kwangju 500757, South Korea Seonam Univ, Dept Biol, Seonam, Namwon, South Korea Seonam Univ Seonam Namwon South Korea Biol, Seonam, Namwon, South Korea Univ Cape Town, Sch Med, MRC, Res Unit Mol Reprod Endocrinol, ZA-7925 CapeTown, South Africa Univ Cape Town Cape Town South Africa ZA-7925 925 CapeTown, South Africa MRC, Reprod Biol Unit, Edinburgh EH9 3ET, Midlothian, Scotland MRC Edinburgh Midlothian Scotland EH9 3ET h EH9 3ET, Midlothian, Scotland
Titolo Testata:
MOLECULAR AND CELLULAR ENDOCRINOLOGY
fascicolo: 1-2, volume: 164, anno: 2000,
pagine: 197 - 204
SICI:
0303-7207(20000620)164:1-2<197:MCDAPC>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXPRESSION PATTERN; RECEPTOR-BINDING; LAMPREY BRAIN; 3 FORMS; I GENE; DISTINCT; IDENTIFICATION; PROTOCHORDATE; LOCALIZATION; SPECIFICITY;
Keywords:
gonadotropin releasing hormone; GnRH; amphibia; cDNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Kwon, HB Chonnam Natl Univ, Hormone Res Ctr, Kwangju 500757, South Korea Chonnam Natl Univ Kwangju South Korea 500757 00757, South Korea
Citazione:
M.S. Yoo et al., "Molecular cloning, distribution and pharmacological characterization of a novel gonadotropin-releasing hormone ([Trp(8)] GnRH) in frog brain", MOL C ENDOC, 164(1-2), 2000, pp. 197-204

Abstract

To date nine structural variants of GnRH have been identified in vertebrates and two additional forms have been isolated from a tunicate. In amphibians only mammalian GnRH ([Arg(8)] GnRH) and type II GnRH (chicken GnRH II, [His(5), Trp(7), Tyr(8)] GnRH) have been identified. In the present study, afull-length cDNA encoding a novel type of GnRH was isolated from pituitaryof Rana dybowskii. The GnRH gene encodes a GnRH peptide ([Trp(8)] GnRH) inwhich tryptophan is substituted for arginine of mammalian GnRH Northern blot analysis revealed the presence of a single 500 bp transcript for the [Trp(8)] GnRH precursor in forebrain but its absence in testis, ovary, kidney and liver. Restriction digests of genomic DNA demonstrated a single copy ofthe gene. The [Trp(8)] GnRH immunoreactive cells were identified in the preoptic area of the frog brain. Synthetic [Trp(8)] GnRH was tested for its ability to stimulate inositol phosphate production by COS-I cells transfected with the cloned Xenopus pituitary GnRH receptor and the cloned human GnRHreceptor. [Trp(8)] GnRH had a potency of about 60% compared with mammalianGnRH ([Arg(8)] GnRH) for the Xenopus receptor, whereas the potency of [Trp(8)] GnRH was approximate to 5% compared with mammalian GnRH for the human receptor. Both mammalian GnRH and [Trp(8)] GnRH were 1000-fold less potent than type II GnRH for the Xenopus GnRH receptor. The similar potency of [Arg(8)] GnRH and the novel [Trp(8)] GnRH for the Xenopus pituitary receptor indicates that, unlike the human receptor, the Xenopus receptor does not discriminate between these amino acids in position eight thereby allowing substitution of the arginine in the mammalian GnRH. (C) 2000 Elsevier Science Ireland Ltd, All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 23:53:17