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Titolo:
Sequence variability of Borna disease virus: Resistance to superinfection may contribute to high genome stability in persistently infected cells
Autore:
Formella, S; Jehle, C; Sauder, C; Staeheli, P; Schwemmle, M;
Indirizzi:
Univ Freiburg, Dept Virol, Inst Med Microbiol & Hyg, D-79104 Freiburg, Germany Univ Freiburg Freiburg Germany D-79104 & Hyg, D-79104 Freiburg, Germany
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 17, volume: 74, anno: 2000,
pagine: 7878 - 7883
SICI:
0022-538X(200009)74:17<7878:SVOBDV>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL-NERVOUS-SYSTEM; RNA VIRUS; DISORDERS; PATHOGENESIS; REPLICATION; EXPRESSION; BLOOD; FORM; LINE; MICE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Schwemmle, M Univ Freiburg, Dept Virol, Inst Med Microbiol & Hyg, Hermann Herder Str 11, D-79104 Freiburg, Germany Univ Freiburg Hermann Herder Str 11 Freiburg Germany D-79104
Citazione:
S. Formella et al., "Sequence variability of Borna disease virus: Resistance to superinfection may contribute to high genome stability in persistently infected cells", J VIROLOGY, 74(17), 2000, pp. 7878-7883

Abstract

The RNA genome of Borna disease virus (BDV) shows extraordinary stability in persistently infected fell cultures, We performed bottleneck experimentsin which virus populations from single infected cells were allowed to spread through cultures of uninfected cells and in which RNase protection assays were used to identify virus variants with mutations in a 535-nucleotide fragment of the M-G open reading frames, In one of the cell cultures, the major virus species (designated 2/1) was a variant with two point mutations in the G open reading frame. When fresh cells were infected with a low dose of a virus stock prepared front 2/1-containing cells, only a minority of the resulting persistently infected cultures contained detectable levels of the variant, whereas the others all seemed to contain wild-type virus. The BDV variant 2/1 remained stable in the various persistently infected cell cultures, indicating that the cells were resistant to superinfection by wild-type virus. Indeed, cells persistently infected with prototype BDV He/80 were also found to resist superinfection with strain V and vice versa, Our screen for mutations in the viral M and G genes of different rat-derived BDV virus stocks revealed that only one of four stocks believed to contain He/80 harbored virus with the original sequence. Two stocks mainly contained a novel virus variant with about 3% sequence divergence, whereas the fourth stock contained a mixture of both viruses, When the mixture was inoculated into the brains of newborn mice, the novel variant was preferentially amplified, These results provide evidence that the BDV genome is mutating more frequently than estimated from its invariant appearance in persistently infected cell cultures and that resistance to superinfection might strongly select against novel variants.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 07:12:14