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Titolo:
Extracellular ATP triggers tumor necrosis factor-alpha release from rat microglia
Autore:
Hide, I; Tanaka, M; Inoue, A; Nakajima, K; Kohsaka, S; Inoue, K; Nakata, Y;
Indirizzi:
Hiroshima Univ, Sch Med, Inst Pharmaceut Sci, Dept Pharmacol,Minami Ku, Hiroshima 7348551, Japan Hiroshima Univ Hiroshima Japan 7348551 nami Ku, Hiroshima 7348551, Japan Natl Inst Neurosci, Dept Neurochem, Tokyo, Japan Natl Inst Neurosci Tokyo Japan t Neurosci, Dept Neurochem, Tokyo, Japan Natl Inst Hlth Sci, Div Pharmacol, Tokyo 158, Japan Natl Inst Hlth Sci Tokyo Japan 158 Sci, Div Pharmacol, Tokyo 158, Japan Kyushu Univ, Grad Sch Pharmaceut Sci, Fukuoka 812, Japan Kyushu Univ Fukuoka Japan 812 rad Sch Pharmaceut Sci, Fukuoka 812, Japan
Titolo Testata:
JOURNAL OF NEUROCHEMISTRY
fascicolo: 3, volume: 75, anno: 2000,
pagine: 965 - 972
SICI:
0022-3042(200009)75:3<965:EATTNF>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
PURINERGIC P2Z RECEPTOR; PROTEIN-KINASE-C; NF-KAPPA-B; CELL-DEATH; GENE-EXPRESSION; BRAIN INJURY; MICE LACKING; ACTIVATION; TNF; PURINORECEPTOR;
Keywords:
microglia; tumor necrosis factor-alpha; ATP; P2X(7) receptor; Ca2+; mitogen-activated protein kinase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Hide, I Hiroshima Univ, Sch Med, Inst Pharmaceut Sci, Dept Pharmacol,Minami Ku, 1-2-3 Kasumi, Hiroshima 7348551, Japan Hiroshima Univ 1-2-3 Kasumi Hiroshima Japan 7348551 348551, Japan
Citazione:
I. Hide et al., "Extracellular ATP triggers tumor necrosis factor-alpha release from rat microglia", J NEUROCHEM, 75(3), 2000, pp. 965-972

Abstract

Brain microglia are a major source of inflammatory cytokines, such as tumor necrosis factor-alpha (TNF-alpha), which have been implicated in the progression of neurodegenerative diseases. Recently, microglia were revealed tobe highly responsive to ATP, which is released from nerve terminals, activated immune cells, or damaged cells. It is not clear, however, whether released ATP can regulate TNF-alpha secretion from microglia. Here we demonstrate that ATP potently stimulates TNF-alpha release, resulting from TNF-alphamRNA expression in rat cultured brain microglia, The TNF-alpha release wasmaximally elicited by 1 mM ATP and also induced by a P2X(7) receptor-selective agonist, 2'- and 3'-O-(4-benzoylbenzoyl)adenosine S'-triphosphate, suggesting the involvement of P2X(7) receptor. ATP-induced TNF-alpha release was Ca2+-dependent, and a sustained Ca2+ influx correlated with the TNF-alpha release in ATP-stimulated microglia, ATP-induced TNF-alpha release was inhibited by PD 098059, an inhibitor of extracellular signal-regulated protein kinase (ERK) kinase 1 (MEK1), which activates ERK, and also by SE 203580,an inhibitor of p38 mitogen-activated protein kinase, ATP rapidly activated both ERK and p38 even in the absence of extracellular Ca2+. These resultsindicate that extracellular ATP triggers TNF-alpha release in rat microglia via a P2 receptor, likely to be the P2X(7) subtype, by a mechanism that is dependent on both the sustained Ca2+ influx and ERK/p38 cascade, regulated independently of Ca2+ influx.

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Documento generato il 02/12/20 alle ore 03:49:55