Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Reciprocal interactions between perisynaptic Schwann cells and regenerating nerve terminals at the frog neuromuscular junction
Autore:
Koirala, S; Qiang, HH; Ko, CP;
Indirizzi:
Univ So Calif, Dept Biol Sci, Neurobiol Sect, Los Angeles, CA 90089 USA Univ So Calif Los Angeles CA USA 90089 ol Sect, Los Angeles, CA 90089 USA
Titolo Testata:
JOURNAL OF NEUROBIOLOGY
fascicolo: 3, volume: 44, anno: 2000,
pagine: 343 - 360
SICI:
0022-3034(20000905)44:3<343:RIBPSC>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
SYNAPTIC EXTRACELLULAR-MATRIX; IN-VIVO OBSERVATIONS; SYNAPSIN-I; MUSCLE-FIBERS; GLIAL-CELLS; PEANUT AGGLUTININ; SCIATIC-NERVE; LIVING MICE; REINNERVATION; LOCALIZATION;
Keywords:
regeneration; glia; nerve terminal; reinnervation; axonal guidance;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Ko, CP Univ So Calif, Dept Biol Sci, Neurobiol Sect, Los Angeles, CA 90089USA Univ So Calif Los Angeles CA USA 90089 , Los Angeles, CA 90089 USA
Citazione:
S. Koirala et al., "Reciprocal interactions between perisynaptic Schwann cells and regenerating nerve terminals at the frog neuromuscular junction", J NEUROBIOL, 44(3), 2000, pp. 343-360

Abstract

The perisynaptic Schwann cell (PSC) has gained recent attention with respect to its roles in synaptic function, remodeling, and regeneration at the vertebrate neuromuscular junction (NMJ). Here we test the hypothesis that, following nerve injury, processes extended by PSCs guide regenerating nerve terminals (NTs) in vivo, and that the extension of sprouts by PSCs is triggered by the arrival of regenerating NTs. Frog NMJs were double-stained witha fluorescent dye, FM4-64, for NTs, and fluorescein isothiocyanate (FITC)-tagged peanut agglutinin (PNA) for PSCs. Identified NMJs were imaged in vivo repeatedly for several months after nerve injury. PSCs sprouted profuselybeginning 3-4 weeks after nerve transection and, as reinnervation progressed, regenerating NTs closely followed the preceding PSC sprouts, which could extend tens to hundreds of microns beyond the original synaptic site. Thepattern of reinnervation was dictated by PSC sprouts, which could form novel routes joining neighboring junctions or develop into new myelinated axonal pathways. In contrast to mammals, profuse PSC sprouting in frog muscles was not seen in response to axotomy alone, and did not occur at chronicallydenervated NMJs. Instead, sprouting coincided with the arrival of regenerating NTs. Immunofluorescent staining revealed that in muscle undergoing reinnervation 4 weeks after axotomy, 91% of NMJs bore PSC sprouts, compared toonly 6% of NMJs in muscle that was chronically denervated for 4 weeks. These results suggest that reciprocal interactions between regenerating NTs and PSCs govern the process of reinnervation at frog NM;ls: regenerating NTs induce PSCs to sprout, and PSG sprouts, in turn, lead and guide the elaboration of NTs. (C) 2000 John Wiley & Sons, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 15:00:42