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Titolo:
Inhibiting proteasome activity causes overreplication of DNA and blocks entry into mitosis in sea urchin embryos
Autore:
Kawahara, H; Philipova, R; Yokosawa, H; Patel, R; Tanaka, K; Whitaker, M;
Indirizzi:
Univ Newcastle Upon Tyne, Sch Med, Dept Physiol Sci, Newcastle Upon Tyne NE2 4HH, Tyne & Wear, England Univ Newcastle Upon Tyne Newcastle Upon Tyne Tyne & Wear England NE2 4HH Hokkaido Univ, Fac Pharmaceut Sci, Sapporo, Hokkaido 060, Japan Hokkaido Univ Sapporo Hokkaido Japan 060 ci, Sapporo, Hokkaido 060, Japan Tokyo Metropolitan Inst Med Sci, Bunkyo Ku, Tokyo 113, Japan Tokyo Metropolitan Inst Med Sci Tokyo Japan 113 kyo Ku, Tokyo 113, Japan
Titolo Testata:
JOURNAL OF CELL SCIENCE
fascicolo: 15, volume: 113, anno: 2000,
pagine: 2659 - 2670
SICI:
0021-9533(200008)113:15<2659:IPACOO>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-CYCLE CONTROL; FISSION YEAST; S-PHASE; PROTEIN-KINASE; SACCHAROMYCES-CEREVISIAE; REPLICATION ORIGINS; TYROSINE PHOSPHORYLATION; ANAPHASE TRANSITION; CATALYTIC SUBUNIT; 26S PROTEASOME;
Keywords:
proteasome; mitosis; p34(cdc2) kinase; DNA synthesis; sea urchin embryo;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
89
Recensione:
Indirizzi per estratti:
Indirizzo: Whitaker, M Univ Newcastle Upon Tyne, Sch Med, Dept Physiol Sci, Framlington Pl, Newcastle Upon Tyne NE2 4HH, Tyne & Wear, England Univ Newcastle Upon Tyne Framlington Pl Newcastle Upon Tyne Tyne & Wear England NE2 4HH
Citazione:
H. Kawahara et al., "Inhibiting proteasome activity causes overreplication of DNA and blocks entry into mitosis in sea urchin embryos", J CELL SCI, 113(15), 2000, pp. 2659-2670

Abstract

The proteasome has been shown to be involved in exit from mitosis by bringing about destruction of mitotic cyclins, Here, we present evidence that the proteasome is also required for proper completion of S phase and for entry into mitosis in the sea urchin embryonic cleavage cycle. A. series of structurally related peptide-aldehydes prevent nuclear envelope breakdown in their order of inhibitory efficacies against the proteasome. Their efficacies in blocking exit from S phase and exit from mitosis correlate well, indicating that the proteasome is involved at both these steps. Mitotic histone HI kinase activation and tyrosine dephosphorylation of p34(cdc2) kinase areblocked by inhibition of the proteasome, indicating that the proteasome plays an important role in the pathway that leads to embryonic p34(cdc2) kinase activation. Arrested embryos continued to incorporate [H-3]thymidine andcharacteristically developed large nuclei. Pre-mitotic arrest can be overcome by treatment with caffeine, a manoeuvre that is known to override the DNA replication checkpoint, These data demonstrate that the proteasome is involved in the control of termination of S phase and consequently in the initiation of M phase of the first embryonic cell cycle.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 14:25:39