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Titolo:
Y-27632, an inhibitor of Rho-associated protein kinase, suppresses tumor cell invasion via regulation of focal adhesion and focal adhesion kinase
Autore:
Imamura, F; Mukai, M; Ayaki, M; Akedo, H;
Indirizzi:
Osaka Med Ctr Canc & Cardiovasc Dis, Dept Pulm Oncol, Higashinari Ku, Osaka 5378511, Japan Osaka Med Ctr Canc & Cardiovasc Dis Osaka Japan 5378511 a 5378511, Japan Osaka Med Ctr Canc & Cardiovasc Dis, Dept Tumor Biochem, Higashinari Ku, Osaka 5378511, Japan Osaka Med Ctr Canc & Cardiovasc Dis Osaka Japan 5378511 a 5378511, Japan Hyogo Coll Med, Dept Pathol 1, Nishinomiya, Hyogo 6638501, Japan Hyogo Coll Med Nishinomiya Hyogo Japan 6638501 miya, Hyogo 6638501, Japan
Titolo Testata:
JAPANESE JOURNAL OF CANCER RESEARCH
fascicolo: 8, volume: 91, anno: 2000,
pagine: 811 - 816
SICI:
0910-5050(200008)91:8<811:YAIORP>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
ASCITES HEPATOMA-CELLS; ACTIN STRESS FIBERS; NA-H EXCHANGE; LYSOPHOSPHATIDIC-ACID; LIM-KINASE; PHOSPHORYLATION; RAC; REORGANIZATION; CYTOSKELETON; DOWNSTREAM;
Keywords:
invasion; ROCK; Y-27632; focal adhesion; focal adhesion kinase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Imamura, F Osaka Med Ctr Canc & Cardiovasc Dis, Dept Pulm Oncol, Higashinari Ku, 1-3-3 Nakamichi, Osaka 5378511, Japan Osaka Med Ctr Canc & Cardiovasc Dis 1-3-3 Nakamichi Osaka Japan 5378511
Citazione:
F. Imamura et al., "Y-27632, an inhibitor of Rho-associated protein kinase, suppresses tumor cell invasion via regulation of focal adhesion and focal adhesion kinase", JPN J CANC, 91(8), 2000, pp. 811-816

Abstract

Migration of rat ascites hepatoma (MM1) cells, invasion and phagokinetic movement were induced by the combination of lysophosphatidic acid (LPA) and fibronectin (FN), Induction of migratory activity was tightly correlated with morphological change of MML cells from spherical or polygonal-shaped cells to fusiform-shaped ones with pseudopodia, MM1 cells were mobile in a fusiform shape, whereas those of a spherical or polygonal shape were not. A small GTPase Rho and one of its downstream effectors ROCK (Rho-associated coiled-coil forming protein kinase), play essential roles in these processes, as evidenced by suppression of migration and morphological change of MM1 cells by Clostridium botulinum C3 exoenzyme, an inhibitor of Rho, or by Y-27632, an inhibitor of ROCK, Y-27632 also suppressed the formation of fusiform-shaped pseudopodia-carrying MM1 cells that was induced by stimulation withthe combination of LPA and PN, LPA and FN also evoked the formation of focal adhesions and actin bundles, and tyrosine phosphorylation of focal adhesion kinase (FAK) and paxillin, The inhibitory effect of Y-27632 on LPA-induced migration and morphological change of MM1 cells was considered to be mediated, at least in part, by impaired formation of focal adhesions and actin bundles. Y-27632 suppressed LPA-induced tyrosine phosphorylation of FAK and paxillin, suggesting that ROCK regulates these molecules and Y-27632 inhibits cellular migration and morphological change, at least in part, through this regulation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 18:24:13