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Titolo:
Preparation and characterization of antibodies against human ribosomal proteins: Heterogeneous expression of S11 and S30 in a panel of human cancer cell lines
Autore:
Nadano, D; Ishihara, G; Aoki, C; Yoshinaka, T; Irie, S; Sato, TA;
Indirizzi:
RIKEN, Mol Oncol Lab, Tsukuba, Ibaraki 3050074, Japan RIKEN Tsukuba Ibaraki Japan 3050074 Lab, Tsukuba, Ibaraki 3050074, Japan Columbia Univ Coll Phys & Surg, Dept Otolaryngol Head & Neck Surg & Pathol, Div Mol Oncol, New York, NY 10032 USA Columbia Univ Coll Phys & Surg NewYork NY USA 10032 w York, NY 10032 USA
Titolo Testata:
JAPANESE JOURNAL OF CANCER RESEARCH
fascicolo: 8, volume: 91, anno: 2000,
pagine: 802 - 810
SICI:
0910-5050(200008)91:8<802:PACOAA>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
T-LYMPHOMA CELLS; GENE-EXPRESSION; FAU CDNA; DROSOPHILA; SYSTEM; IDENTIFICATION; TRANSLATION; SUPPRESSION; APOPTOSIS; SEQUENCE;
Keywords:
proteomics; ribosomal proteins; antibodies; expression profiles; human cancer;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Sato, TA RIKEN, Mol Oncol Lab, Tsukuba, Ibaraki 3050074, Japan RIKEN Tsukuba Ibaraki Japan 3050074 kuba, Ibaraki 3050074, Japan
Citazione:
D. Nadano et al., "Preparation and characterization of antibodies against human ribosomal proteins: Heterogeneous expression of S11 and S30 in a panel of human cancer cell lines", JPN J CANC, 91(8), 2000, pp. 802-810

Abstract

Mutants of model eukaryotic organisms have revealed that most ribosomal proteins are essential for cell viability. However, the precise functional role of each ribosomal protein is largely unknown, Recent reports on the involvement of ribosomal proteins in various genetic diseases and studies on the extraribosomal functions of these proteins have cast some light on their localization and functions, Here we prepared rabbit polyclonal antibodies against 26 human ribosomal proteins; each of these reagents recognized a single band in immunoblots of the purified ribosome, We used these antibodies to evaluate a panel of human cancer cell lines. Although no deficiency of ribosomal proteins was observed, the abundance of S11 and S30 varied substantially among the cell lines, but the difference did not affect the biogenesis or composition of the ribosome, Therefore, the heterogeneity may be related to extraribosomal functions of S11 and S30, The antibodies described here are powerful tools for research into the molecular mechanisms of proteintranslation, cell-biological and medical studies on the ribosomal proteins, and ultimately a comprehensive understanding of all ribosomal proteins ("ribosomics").

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 04:44:33