Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Human response to Escherichia coli O157 : H7 infection: Antibodies to secreted virulence factors
Autore:
Li, YL; Frey, E; Mackenzie, AMR; Finlay, BB;
Indirizzi:
Univ British Columbia, Biotechnol Lab, Vancouver, BC V6T 1Z3, Canada Univ British Columbia Vancouver BC Canada V6T 1Z3 ver, BC V6T 1Z3, Canada Div Microbiol, Ottawa, ON K1Y 4E9, Canada Div Microbiol Ottawa ON Canada K1Y 4E9 robiol, Ottawa, ON K1Y 4E9, Canada
Titolo Testata:
INFECTION AND IMMUNITY
fascicolo: 9, volume: 68, anno: 2000,
pagine: 5090 - 5095
SICI:
0019-9567(200009)68:9<5090:HRTECO>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMOLYTIC-UREMIC SYNDROME; HOST EPITHELIAL-CELLS; SERUM ANTIBODIES; PROTEIN TRANSLOCATION; LIPOPOLYSACCHARIDE; ESPA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Finlay, BB Univ British Columbia, Biotechnol Lab, Room 237,Wesbrook Bldg,6174 Univ Blvd, Vancouver, BC V6T 1Z3, Canada Univ British Columbia Room 237,Wesbrook Bldg,6174 Univ Blvd Vancouver BC Canada V6T 1Z3
Citazione:
Y.L. Li et al., "Human response to Escherichia coli O157 : H7 infection: Antibodies to secreted virulence factors", INFEC IMMUN, 68(9), 2000, pp. 5090-5095

Abstract

Vaccination has been proposed for the prevention of disease due to enterohemorrhagic Escherichia coli (EHEC), but the immune response following humaninfection, including the choice of potential antigens, has not been well characterized. To study this, sera were obtained from five pediatric patients with acute diarrhea caused by E. coli O157:117 0, 8, and 60 days after hospitalization. These sera were used to examine the immune response to four different EHEC virulence factors: Tir (translocated intimin receptor, whichis inserted into the host cell membrane), intimin (bacterial outer membrane protein which binds to Tir), EspA (secreted protein which forms filamentous structures on EHEC surface), and EspB (inserted into the host membrane and cytoplasm). The response to O157:H7 lipopolysaccharide was also examined. Sera were assayed against purified recombinant proteins using immunoblot analysis and by enzyme-linked immunosorbent assay to determine the sera's titers to each of the antigens in all patients, We found that there was little reaction to EspA EspB, and intimin in the acute-phase sera, although there was some reactivity to Tir. By day 8, titers of antibody to all four virulence factors were present in all patients, with a very strong response against Tir (up to a titer of 1:256,000), especially in hemolytic-uremic syndrome patients, and lesser strong responses to the other three antigens. Thetiter to the antigens 60 days after hospitalization was decreased but was still highest for Tir. These results suggest that there is a strong immune response to Tir, and to a lesser extent to the other three virulence factors, following EHEC disease, indicating that these bacterial molecules are potential vaccine candidates for preventing EHEC disease. They also suggest that bacterial virulence factors that are inserted into host cells during infection by type III secretion systems (Tir or EspB) are still recognized bythe host immune response.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 05:07:05