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Titolo:
A novel factor isolated from Actinobacillus actinomycetemcomitans stimulates mouse B cells and human peripheral blood mononuclear cells
Autore:
Jeong, SJ; Yee, ST; Jo, WS; Yu, SH; Lee, SH; Lim, YJ; Yoo, YH; Kim, JM; Lee, JD; Jeong, MH;
Indirizzi:
Dong A Univ, Coll Med, Inst Med Sci, Pusan 600103, South Korea Dong A Univ Pusan South Korea 600103 Med Sci, Pusan 600103, South Korea Dong A Univ, Coll Med, Dept Clin Pathol, Pusan, South Korea Dong A Univ Pusan South Korea Med, Dept Clin Pathol, Pusan, South Korea Pusan Natl Univ, Dept Microbiol, Pusan 609735, South Korea Pusan Natl Univ Pusan South Korea 609735 biol, Pusan 609735, South Korea Sunchon Natl Univ, Dept Biol, Sunchon, South Korea Sunchon Natl Univ Sunchon South Korea , Dept Biol, Sunchon, South Korea
Titolo Testata:
INFECTION AND IMMUNITY
fascicolo: 9, volume: 68, anno: 2000,
pagine: 5132 - 5138
SICI:
0019-9567(200009)68:9<5132:ANFIFA>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERIODONTAL-DISEASE; T-CELLS; LYMPHOCYTE-TRANSFORMATION; ADULT PERIODONTITIS; BACTERIAL-ANTIGENS; SUPPRESSION; EXPRESSION; RESPONSES; TISSUES; LIPOPOLYSACCHARIDE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Jeong, MH Dong A Univ, Coll Med, Dept Microbiol, Pusan 600103, South KoreaDong A Univ Pusan South Korea 600103 Pusan 600103, South Korea
Citazione:
S.J. Jeong et al., "A novel factor isolated from Actinobacillus actinomycetemcomitans stimulates mouse B cells and human peripheral blood mononuclear cells", INFEC IMMUN, 68(9), 2000, pp. 5132-5138

Abstract

A novel immunostimulating factor (ISTF) Actinobacillus actinomycetemcomitans ATCC 29522 was isolated and characterized as inducing proliferation of mouse B cells and human peripheral blood mononuclear cells. This factor was isolated from the bacterial culture medium and purified by size exclusion chromatography, dye-ligand affinity chromatography, immunoaffinity chromatography using monoclonal antibodies, and preparative electrophoresis. Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that the purified ISTF migrated as a single band corresponding to a molecular mass of 13 kDa. ISTF was a proteinaceous material distinct from lipopolysaccharide; it directly induced the proliferation of B lymphocytes but had no effect on the proliferation of T lymphocytes, even in the presence of antigen-presenting cells. A B-lymphocyte-mitogenic activity of ISTF was also shown by flow cytometric analysis of responding cell sub-populations. Immunoblot analysis revealed that ISTF was a component of the outer membranes of bacteria, could exist as a soluble form, and was released by growing and/or lysed bacteria. These results suggest that ISTF produced by A. actinomycetemcomitans may play an important role in immunopathologic changes associated with A. actinomycetemcomitans infections.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/07/20 alle ore 05:54:26