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Titolo:
Expression of matrix metalloproteinases and related tissue inhibitors in the cyst fluids of ovarian mucinous neoplasms
Autore:
Furuya, M; Ishikura, H; Kawarada, Y; Ogawa, Y; Sakuragi, N; Fujimoto, S; Yoshiki, T;
Indirizzi:
Hokkaido Univ, Sch Med, Dept Pathol, Sapporo, Hokkaido 060, Japan HokkaidoUniv Sapporo Hokkaido Japan 060 ol, Sapporo, Hokkaido 060, Japan Hokkaido Univ, Sch Med, Dept Obstet & Gynecol, Sapporo, Hokkaido 060, Japan Hokkaido Univ Sapporo Hokkaido Japan 060 ol, Sapporo, Hokkaido 060, Japan Chiba Univ, Sch Med, Dept Pathol, Chuo Ku, Chiba 2608670, Japan Chiba Univ Chiba Japan 2608670 ept Pathol, Chuo Ku, Chiba 2608670, Japan
Titolo Testata:
GYNECOLOGIC ONCOLOGY
fascicolo: 2, volume: 78, anno: 2000,
pagine: 106 - 112
SICI:
0090-8258(200008)78:2<106:EOMMAR>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
IV COLLAGENASE; DEGRADING PROTEINASES; CARCINOMA-CELLS; GELATINASE-A; INVASION; TUMORS; IDENTIFICATION; PURIFICATION; METASTASIS; MATRILYSIN;
Keywords:
ovary; mucinous tumor; cystic fluid; matrix metalloproteinase (MMP); trypsin; tissue inhibitor of metalloproteinase (TIMP);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Ishikura, H Chiba Univ, Sch Med, Dept Pathol, Chuo Ku, Inohana 1-8-1, Chiba 2608670, Japan Chiba Univ Inohana 1-8-1 Chiba Japan 2608670 a 2608670, Japan
Citazione:
M. Furuya et al., "Expression of matrix metalloproteinases and related tissue inhibitors in the cyst fluids of ovarian mucinous neoplasms", GYNECOL ONC, 78(2), 2000, pp. 106-112

Abstract

Objectives and methods. The growth of an ovarian cystic neoplasm often involves its invasion into and destruction of the extracellular matrix. We examined neoplastic cysts of ovarian mucinous tumors for the presence of matrix metalloproteinases (MMPs) and tissue inhibitors of MMPs (TIMPs) using zymography (in situ zymography, gelatin zymography, and casein zymography) andenzyme-linked immunosorbent assay. Results. Matriolytic activity was detected within the cystic contents and cytoplasm of the lining epithelial cells of the cyst by in situ zymography. This intracystic matriolytic activity was thought to originate mainly in the epithelial cells. The activated form of MMP-9 was: seen in all carcinomaand borderline fluids and in 7 of 15 adenomas. The concentration of MMP-9 was higher in carcinoma fluids than in borderline and adenoma fluids (P < 0.05). TIMP-1, which specifically binds to MMP-9, was also higher in carcinoma and borderline fluids than in adenoma fluids (P < 0.05). MMP-2 activity was nearly ubiquitously present in all cyst fluids, irrespective of the fluid's histologic category. The amount of MMP-2 was highest in the carcinoma category, although not to a statistically significant degree. TIMP-2, a specific inhibitor for MMP-2, was significantly lower in the borderline category than in the adenoma category. The molar ratios of TIMP-1/MMP-9 (not significant) and TIMP-2/MMP-2 (P < 0.05) were higher in the adenoma category. Expressions of trypsin, MMP-7, and MMP-9 were generally higher in carcinoma and borderline fluids than in adenoma fluids. Conclusions. These observations indicate the importance of ovarian cystic fluids for analyzing tumor-associated matriolytic activities. The findings suggest that these matriolytic enzymes, together with the presence of theirinhibitors, play an important role in the growth of ovarian mucinous tumors. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 16:09:31