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Titolo:
Role of fucosyltransferases in the association between apomucin and Lewis antigen expression in normal and malignant gastric epithelium
Autore:
Lopez-Ferrer, A; de Bolos, C; Barranco, C; Garrido, M; Isern, J; Carlstedt, I; Reis, CA; Torrado, J; Real, FX;
Indirizzi:
Univ Pompeu Fabra, Inst Municipal Invest Med, Unitat Biol Cellular & Mol, Barcelona 08003, Spain Univ Pompeu Fabra Barcelona Spain 08003 ar & Mol, Barcelona 08003, Spain Hosp Mar, Serv Patol, Barcelona, Spain Hosp Mar Barcelona SpainHosp Mar, Serv Patol, Barcelona, Spain Univ Lund, Mucosal Biol Grp, S-22100 Lund, Sweden Univ Lund Lund Sweden S-22100 nd, Mucosal Biol Grp, S-22100 Lund, Sweden Univ Porto, Inst Mol Pathol & Immunol, Porto, Portugal Univ Porto Porto Portugal o, Inst Mol Pathol & Immunol, Porto, Portugal Arantzazu Ospitalea, San Sebastian, Spain Arantzazu Ospitalea San Sebastian Spain Ospitalea, San Sebastian, Spain
Titolo Testata:
GUT
fascicolo: 3, volume: 47, anno: 2000,
pagine: 349 - 356
SICI:
0017-5749(200009)47:3<349:ROFITA>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSCRIPTION START SITES; INTESTINAL MUCIN GENE; BLOOD-GROUP ANTIGENS; MOLECULAR-CLONING; HELICOBACTER-PYLORI; MICROSATELLITE INSTABILITY; DIFFERENTIAL EXPRESSION; MONOCLONAL-ANTIBODY; SURFACE EPITHELIUM; NORMAL TISSUE;
Keywords:
fucosyltransferases; gastric carcinogenesis; gastric mucins; Lewis antigens;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: de Bolos, C Univ Pompeu Fabra, Inst Municipal Invest Med, Unitat Biol Cellular & Mol, Dr Aiguader 80, Barcelona 08003, Spain Univ Pompeu Fabra Dr Aiguader 80 Barcelona Spain 08003 Spain
Citazione:
A. Lopez-Ferrer et al., "Role of fucosyltransferases in the association between apomucin and Lewis antigen expression in normal and malignant gastric epithelium", GUT, 47(3), 2000, pp. 349-356

Abstract

Background-In normal gastric epithelium, MUC5AC is detected in superficialepithelium associated with Lewis type 1 antigens and MUC6 is detected in antral glands with Lewis type 2. Therefore, the stomach constitutes an excellent model to examine the role of glycosyltransferases in determining the specificity of apomucin glycosylation. Aims-To determine the molecular basis of this association and to examine changes in expression of gastric and intestinal apomucins and their association with Lewis antigens during the gastric carcinogenesis process. Methods-Fucosyltransferase (FUT1, FUT2, FUT3) and mucin (MUC5AC, MUC6) transcripts were detected using reverse transcription-polymerase chain reaction. Apomucin (MUC2, MUC4, MUC5AC, MUC6) and Lewis antigen (types 1 and 2) expression were analysed using single and double immunohistochemistry and in situ hybridisation. Results-In the normal stomach, FUT1 is exclusively detected associated with MUC6; FUT2 is only detected when MUC5AC is present. This co-regulation islost in gastric tumours, as is differential expression of MUC5AC and MUC6 in normal gastric epithelial cells. In gastric tumours, especially those with the intestinal phenotype, MUC2 and MUC4 genes are upregulated, and gastric-type and intestinal-type mucins are coexpressed. These changes are earlyevents in the gastric carcinogenesis process, as they are detected in intestinal metaplasia. Conclusions-The glycosylation pattern found in normal gastric epithelium is dictated by the specific set of fucosyltranferases expressed by the cellsrather than by the apomucin sequence. The development of intestinal metaplasia and gastric cancer is associated with the appearance of cellular phenotypes that are absent from normal epithelium.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 12:46:54