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Titolo:
Loss of heterozygosity assay for molecular detection of cancer using energy-transfer primers and capillary array electrophoresis
Autore:
Medintz, IL; Lee, CCR; Wong, WW; Pirkola, K; Sidransky, D; Mathies, RA;
Indirizzi:
Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA Univ Calif Berkeley Berkeley CA USA 94720 pt Chem, Berkeley, CA 94720 USA Johns Hopkins Univ, Sch Med, Div Head & Neck Canc Res, Dept Otolaryngol Head & Neck Surg, Baltimore, MD 21205 USA Johns Hopkins Univ Baltimore MD USA 21205 k Surg, Baltimore, MD 21205 USA Mol Dynam, Sunnyvale, CA 94086 USA Mol Dynam Sunnyvale CA USA 94086Mol Dynam, Sunnyvale, CA 94086 USA
Titolo Testata:
GENOME RESEARCH
fascicolo: 8, volume: 10, anno: 2000,
pagine: 1211 - 1218
SICI:
1088-9051(200008)10:8<1211:LOHAFM>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
MICROSATELLITE ANALYSIS; BLADDER-CANCER; PCR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Mathies, RA Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA Univ Calif Berkeley Berkeley CA USA 94720 keley, CA 94720 USA
Citazione:
I.L. Medintz et al., "Loss of heterozygosity assay for molecular detection of cancer using energy-transfer primers and capillary array electrophoresis", GENOME RES, 10(8), 2000, pp. 1211-1218

Abstract

Microsatellite DNA loci are useful markers For the detection of loss of heterozygosity (LOH) and microsatellite instability (MI) associated with primary cancers. To carry out targe-scale studies of LOH and MI in cancer progression, high-throughput instrumentation and assays with high accuracy and sensitivity need to be validated. DNA was extracted from 26 renal tumor and paired lymphocyte samples and amplified with two-color energy-transfer (ET)fluorescent primers specific for loci associated with cancer-induced chromosomal changes. PCR amplicons were separated on the MegaBACE-1000 96 capillary array electrophoresis (CAE) instrument and analyzed with MegaBACE Genetic Profiler v.1.0 software. Ninety-six separations were achieved in parallel in 75 minutes. Loss of heterozygosity was easily detected in tumor samples as was the gain/loss of microsatellite core repeats. Allelic ratios were determined with a precision of +/- 10% or better. Prior analysis of these samples with slab gel electrophoresis and radioisotope labeling had not detected these changes with as much sensitivity or precision. This study establishes the validity of this assay and the MegaBACE instrument for large-scale, high-throughput studies of the molecular genetic changes associated withcancer.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 07:08:37