Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Liposome-mediated, nonviral gene transfer induces a systemic inflammatory response which can exacerbate pre-existing inflammation
Autore:
Norman, J; Denham, W; Denham, D; Yang, J; Carter, G; Abouhamze, A; Tannahill, CL; MacKay, SLD; Moldawer, LL;
Indirizzi:
Univ S Florida, Coll Med, Dept Surg, Tampa, FL USA Univ S Florida Tampa FL USA Florida, Coll Med, Dept Surg, Tampa, FL USA Univ Florida, Coll Med, Dept Surg, Gainesville, FL USA Univ Florida Gainesville FL USA Coll Med, Dept Surg, Gainesville, FL USA
Titolo Testata:
GENE THERAPY
fascicolo: 16, volume: 7, anno: 2000,
pagine: 1425 - 1430
SICI:
0969-7128(200008)7:16<1425:LNGTIA>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
BACTERIAL-DNA; CPG MOTIFS; LETHAL ENDOTOXEMIA; CHOLINE-DEFICIENT; T-LYMPHOCYTES; PANCREATITIS; NECROSIS; THERAPY; MICE; MECHANISM;
Keywords:
pancreatitis; TNF alpha; IL-1; plasmid DNA; endotoxin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Moldawer, LL Univ Florida, Coll Med, Dept Surg, Shands Hosp, POB 100286,Room 6166,1600 SW Archer Rd, Gainesville, FL 32610 USA Univ Florida POB 100286,Room 6166,1600 SW Archer Rd Gainesville FL USA 32610
Citazione:
J. Norman et al., "Liposome-mediated, nonviral gene transfer induces a systemic inflammatory response which can exacerbate pre-existing inflammation", GENE THER, 7(16), 2000, pp. 1425-1430

Abstract

Cationic liposome and plasmid-mediated gene transfer has emerged as a novel technique for the targeted delivery of protein-based therapies in acute inflammatory diseases. However, concerns have arisen that cationic liposomesand plasmid DNA have inherent proinflammatory properties which could exacerbate pre-existing inflammatory processes. In healthy mice, intraperitonealadministration of cationic liposomes (200 nmol) complexed to plasmid DNA (100 mu g) induced a proinflammatory response characterized by the inductionof tumor necrosis factor alpha and interleukin-1 beta mRNA expression. Theplasma concentrations of the hepatic acute phase proteins interleukin-6, amyloid A, amyloid P, and seromucoid were also increased (P < 0.05), and this response was seen in endotoxin-resistant (C3H/HeJ) mice. The inflammatoryresponse associated with gene transfer increased the mortality and severity of experimentally induced sterile inflammation (pancreatitis). We conclude that systemic administration of cationic liposomes and plasmid DNA is associated with induction of the innate immune response which may exacerbate pre-existing inflammatory processes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 18:02:57