Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Improved titers of HIV-based lentiviral vectors using the SRV-1 constitutive transport element
Autore:
Mautino, MR; Keiser, N; Morgan, RA;
Indirizzi:
NHGRI, Clin Genet Therapy Branch, NIH, Bethesda, MD 20892 USA NHGRI Bethesda MD USA 20892 t Therapy Branch, NIH, Bethesda, MD 20892 USA
Titolo Testata:
GENE THERAPY
fascicolo: 16, volume: 7, anno: 2000,
pagine: 1421 - 1424
SICI:
0969-7128(200008)7:16<1421:ITOHLV>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNODEFICIENCY-VIRUS TYPE-1; GENE DELIVERY; REV FUNCTION; REPLICATION; REQUIREMENT; PROPAGATION; EXPRESSION; PROTEIN; RNA;
Keywords:
rev-independent; lentiviral vector; CTE; gene therapy; HIV/AIDS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Morgan, RA NHGRI, Clin Genet Therapy Branch, NIH, 10 Ctr Dr,Bldg 10,Room 10C103, Bethesda, MD 20892 USA NHGRI 10 Ctr Dr,Bldg 10,Room 10C103 Bethesda MD USA 20892 2 USA
Citazione:
M.R. Mautino et al., "Improved titers of HIV-based lentiviral vectors using the SRV-1 constitutive transport element", GENE THER, 7(16), 2000, pp. 1421-1424

Abstract

The development of lentiviral vectors that use Rev-independent mechanisms of nuclear export for their genomic RNA could facilitate the construction of novel anti-HIV vectors. We have improved the titers of Rev-independent lentiviral vectors having the SRV-1 CTE by mutating the major splice donor and acceptor sites present in the vector and by relocalization of the CTE sequences adjacent to the HIV-I 3'LTR. These two modifications have additive beneficial effects on vector titers and packaging efficiency. Packaging these CTE+ vectors expressing marker genes with a Rev-dependent HIV-1 helper vector yields higher titers than are obtained using a Rev-dependent lentiviral vector.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 11:22:14