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Titolo:
Evidence that estrogens directly alter androgen-regulated prostate development
Autore:
Jarred, RA; Cancilla, B; Prins, GS; Thayer, KA; Cunha, GR; Risbridger, GP;
Indirizzi:
Monash Univ, Monash Med Ctr, Monash Inst Reprod & Dev, Clayton, Vic 3168, Australia Monash Univ Clayton Vic Australia 3168 Dev, Clayton, Vic 3168, Australia Univ Calif San Francisco, Sch Med, Dept Anat, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Univ Illinois, Coll Med, Dept Urol, Chicago, IL 60612 USA Univ Illinois Chicago IL USA 60612 Med, Dept Urol, Chicago, IL 60612 USA
Titolo Testata:
ENDOCRINOLOGY
fascicolo: 9, volume: 141, anno: 2000,
pagine: 3471 - 3477
SICI:
0013-7227(200009)141:9<3471:ETEDAA>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT VENTRAL PROSTATE; RECEPTOR EXPRESSION; ANTERIOR PROSTATE; SEMINAL-VESICLE; REPRODUCTIVE-ORGANS; EXPOSURE; MICE; DIETHYLSTILBESTROL; GROWTH; GLAND;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Risbridger, GP Monash Univ, Monash Med Ctr, Monash Inst Reprod & Dev, 246 Clayton Rd, Clayton, Vic 3168, Australia Monash Univ 246 Clayton Rd ClaytonVic Australia 3168 ralia
Citazione:
R.A. Jarred et al., "Evidence that estrogens directly alter androgen-regulated prostate development", ENDOCRINOL, 141(9), 2000, pp. 3471-3477

Abstract

Neonatal exposure to high doses of estrogen results in permanent suppression of prostate growth and reduced sensitivity to androgens in adulthood. Itis unclear whether alterations in prostate growth are due to a direct effect of estrogens on the gland or are the result of hypothalamic-pituitary-gonadal axis suppression and a subsequent reduction in androgen levels. Therefore, the aim of this study was to determine whether estrogens have a direct effect on the prostate using a defined method of culturing neonatal prostates. Newborn rat ventral prostates were microdissected and cultured in thepresence of testosterone, which resulted in branching morphogenesis and ductal canalization. Solid cords of epithelium differentiated into acini lined by tall columnar epithelial cells; these acini were surrounded by stromalcells, expressing smooth muscle alpha-actin. When cultured in the presenceof 17 beta-estradiol or diethylstilbestrol in addition to testosterone, androgen-induced prostatic growth was reduced, and differentiation was altered. Although estrogen-treated explants were smaller than controls, quantification of epithelial, stromal, and luminal volumes using unbiased stereologyrevealed significant changes; the proportion of epithelial cells and lumendecreased, and the proportion of stroma increased compared with control values. Concurrent with this reduced growth rate, we observed a disturbance in the branching pattern and a reduction in ductal canalization. Specifically, stromal differentiation and organization were disrupted, so that a discontinuous smooth muscle layer was observed around the epithelial ducts, and epithelial differentiation was altered. The effects of estrogens were not accompanied by a decrease in androgen response via the androgen receptor, because immunolocalization of this receptor remained constant. These data demonstrate that high doses of estrogens are growth inhibitory and have directeffects on prostate development in vitro, which may occur in vivo in addition to indirect effects via suppression of the hypothalamic-pituitary-gonadal axis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 21:31:34