Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Effect of myenteric denervation on intestinal epithelium proliferation andmigration of suckling and weanling rats
Autore:
Hernandes, L; Zucoloto, S; Alvares, EP;
Indirizzi:
Univ Sao Paulo, Inst Ciencias Biomed, Dept Histol & Embryol, BR-05508 Sao Paulo, Brazil Univ Sao Paulo Sao Paulo Brazil BR-05508 yol, BR-05508 Sao Paulo, Brazil Univ Sao Paulo, Fac Med, Dept Pathol, BR-14049 Ribeirao Preto, SP, Brazil Univ Sao Paulo Ribeirao Preto SP Brazil BR-14049 beirao Preto, SP, Brazil
Titolo Testata:
CELL PROLIFERATION
fascicolo: 3, volume: 33, anno: 2000,
pagine: 127 - 138
SICI:
0960-7722(200006)33:3<127:EOMDOI>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
CRYPT CELL-PROLIFERATION; JEJUNAL SMOOTH-MUSCLE; BENZALKONIUM CHLORIDE; CHEMICAL ABLATION; ENTERIC NEURONS; PLEXUS ABLATION; POSTNATAL LIFE; DIFFERENTIATION; SYMPATHECTOMY; DESTRUCTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Alvares, EP Univ Sao Paulo, Inst Ciencias Biomed, Dept Histol & Embryol, Av Lineu Prestes 1524, BR-05508 Sao Paulo, Brazil Univ Sao Paulo Av Lineu Prestes 1524 Sao Paulo Brazil BR-05508
Citazione:
L. Hernandes et al., "Effect of myenteric denervation on intestinal epithelium proliferation andmigration of suckling and weanling rats", CELL PROLIF, 33(3), 2000, pp. 127-138

Abstract

The effects of myenteric denervation on the cell kinetics of the intestinal epithelium of suckling and weanling rats were investigated. The myentericplexus of an ileal segment was partially ablated by serosal application ofbenzalkonium chloride (BAC) in three groups of rats: those that underwent surgery at 13 days and were killed 15 (13/28-day-old) or 23 (13/36-day-old)days after treatment, and those that were operated at 21 days (21/36-day-old) and were killed 15 days after treatment. The extent of denervation was assessed in whole-mount preparations. The cell bodies of myenteric neuroneswere stained by NADH-diaphorase histochemical technique. Cell proliferation was estimated by the mitotic index (MI) and morphometric analysis of villus and crypt lengths using an image analysis system. Thickness of the muscle layers was also assessed by morphometry, Cell migration on the villi was estimated by the position of the leading labelled cell 24 h after tritiatedthymidine injection. The number of neurones was reduced by around 80% in rats operated at 13 days, and reduced by 98% in those operated at 21 days. The thickness of the muscle layers was increased in all groups of treated animals. MI was significantly higher 15 days after BAG-treatment in the 13/28group. Morphological changes in the intestinal mucosa were observed 15 days after BAG-treatment, when there was an increase in villus height (13/28 group) and crypt depth (13/28 and 21/36 groups). Cell migration rate was accelerated in the 21/36 group. No differences where found in the 13/36 group. These results show the strong effect of myenteric ablation on cell proliferation and migration in the ileal epithelium in the first 15 days of treatment in suckling and in weanling rats, and the subsequent recovery of intestinal mucosa homeostasis later on.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 09:55:36