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Titolo:
A comparison between MALDI-TOF mass spectrometry and HPAEC-PAD analysis ofdebranched starch
Autore:
Broberg, S; Koch, K; Andersson, R; Kenne, L;
Indirizzi:
Swedish Univ Agr Sci, Dept Chem, SE-75007 Uppsala, Sweden Swedish Univ AgrSci Uppsala Sweden SE-75007 m, SE-75007 Uppsala, Sweden Swedish Univ Agr Sci, Dept Food Sci, SE-75007 Uppsala, Sweden Swedish UnivAgr Sci Uppsala Sweden SE-75007 i, SE-75007 Uppsala, Sweden
Titolo Testata:
CARBOHYDRATE POLYMERS
fascicolo: 3, volume: 43, anno: 2000,
pagine: 285 - 289
SICI:
0144-8617(200011)43:3<285:ACBMMS>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
ASSISTED LASER-DESORPTION; ANION-EXCHANGE CHROMATOGRAPHY; AMYLOPECTIN UNIT CHAINS; TIME-OF-FLIGHT; QUANTITATIVE-ANALYSIS; MATRIX; OLIGOSACCHARIDES;
Keywords:
starch; quantification; MALDI-TOF MS; HPAEC-PAD; amylopectin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Broberg, S Swedish Univ Agr Sci, Dept Chem, POB 7015, SE-75007 Uppsala, Sweden Swedish Univ Agr Sci POB 7015 Uppsala Sweden SE-75007 , Sweden
Citazione:
S. Broberg et al., "A comparison between MALDI-TOF mass spectrometry and HPAEC-PAD analysis ofdebranched starch", CARBOHY POL, 43(3), 2000, pp. 285-289

Abstract

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, MALDI-TOF MS, was used in this study to determine chain length distribution of amylopectin, the main constituent of starch. The technique was compared with high-performance anion-exchange chromatography with pulsed amperometric detection, HPAEC-PAD, an established technique for this purpose. Starch from potato, wheat, and waxy maize was debranched with isoamylase and analysed using both techniques. MALDI-TOF MS is a faster and more sensitive technique and provides more detailed information on the molecular mass of the unit chains. A difference between chain length distribution for amylopectin from different sources was observed with both methods. In addition, removal of amylose was not necessary for MALDI-TOF analysis. However, the technique was less reproducible than HPAEC-PAD and overestimated the amount of longer chains. (C) 2000 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 01:45:14