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Titolo:
Comparative genomic hybridization analysis of 38 breast cancer cell lines:A basis for interpreting complementary DNA microarray data
Autore:
Forozan, F; Mahlamaki, EH; Monni, O; Chen, YD; Veldman, R; Jiang, Y; Gooden, GC; Ethier, SP; Kallioniemi, A; Kallioniemi, OP;
Indirizzi:
NHGRI, Canc Genet Branch, NIH, Bethesda, MD 20892 USA NHGRI Bethesda MD USA 20892 anc Genet Branch, NIH, Bethesda, MD 20892 USA Tampere Univ, Inst Med Technol, Canc Genet Lab, FIN-33521 Tampere, FinlandTampere Univ Tampere Finland FIN-33521 t Lab, FIN-33521 Tampere, Finland Tampere Univ Hosp, FIN-33521 Tampere, Finland Tampere Univ Hosp Tampere Finland FIN-33521 , FIN-33521 Tampere, Finland Univ Michigan, Sch Med, Dept Radiat Oncol, Div Radiat & Canc Biol, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 & Canc Biol, Ann Arbor, MI 48109 USA
Titolo Testata:
CANCER RESEARCH
fascicolo: 16, volume: 60, anno: 2000,
pagine: 4519 - 4525
SICI:
0008-5472(20000815)60:16<4519:CGHAO3>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
CARCINOMA IN-SITU; GENETIC ABERRATIONS; DIFFERENT PATTERNS; CHROMOSOME 20Q13; CDNA MICROARRAYS; AMPLIFICATION; TUMORS; CGH; ONCOGENE; REVEALS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Kallioniemi, OP NHGRI, Canc Genet Branch, NIH, Bldg 49,Room 4A24,49 Convent Dr,MSC 4470, Bethesda, MD 20892 USA NHGRI Bldg 49,Room 4A24,49 Convent Dr,MSC 4470 Bethesda MD USA 20892
Citazione:
F. Forozan et al., "Comparative genomic hybridization analysis of 38 breast cancer cell lines:A basis for interpreting complementary DNA microarray data", CANCER RES, 60(16), 2000, pp. 4519-4525

Abstract

Breast cancer cell lines provide a useful starting point for the discoveryand functional analysis of genes involved in breast cancer. Here, we studied 38 established breast cancer cell lines by comparative genomic hybridization (CGH) to determine recurrent genetic alterations and the extent to which these cell lines resemble uncultured tumors. The following chromosomal gains were observed: 8q (75%), 1q (61%), 20q (55%), 7p (44%), 3q (39%), 5p (39%), 7q (39%), 17q (33%), 1p (30%), and 20p (30%), and the most common losses were: 8p (58%), 18q (58%), 1p (42%), Xp (42%),,Xq (42%), 4p (36%), 11q (36%), 18p (33%), 10q (30%), and 19p (28%). Furthermore, 35 recurrent high-level amplification sites were identified, most often involving 8q23 (37%),20q13 (29%), 3q25-q26 (24%), 17q22-q23 (16%), 17q23-q24 (16%), 1p13 (11%),1q32 (11%), 5p13 (11%), 5p14 (11%), 11q13 (11%), 17q12-q21(11%), and 7q21-q22 (11%). A comparison of DNA copy number changes found in the cell lines with those reported in 17 published studies (698 tumors) of uncultured tumors revealed a substantial degree of overlap. CGH copy number profiles may facilitate identification of important new genes located at the hotspots of such chromosomal alterations. This was illustrated by analyzing expression levels of 1236 genes using cDNA microarrays in four of the cell lines. Several highly overexpressed genes (such as RCH1 at 17q23, TOPO II at 17q21-q22, as well as CAS and MYBL2 at 20q13) were involved in these recurrent DNA amplifications. In conclusion, DNA copy number profiles were generated by CGH for most of the publicly available breast cancer cell lines and mere made available on a web site (http://www.nhgri.nih.gov/DIR/CGB/CR2000). This should facilitate the correlative analysis of gene expression and copy number as illustrated here by the finding by cDNA microarrays of several overexpressed genes that were amplified.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 20:49:12