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Titolo:
ATP-induced [Ca2+](i) changes and depolarization in GH3 cells
Autore:
Chung, HS; Park, KS; Cha, SK; Kong, ID; Lee, JW;
Indirizzi:
Yonsei Univ, Wonju Coll Med, Dept Physiol, Wonju 220701, South Korea Yonsei Univ Wonju South Korea 220701 Physiol, Wonju 220701, South Korea
Titolo Testata:
BRITISH JOURNAL OF PHARMACOLOGY
fascicolo: 8, volume: 130, anno: 2000,
pagine: 1843 - 1852
SICI:
0007-1188(200008)130:8<1843:A[CADI>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
P(2Z) PURINERGIC RECEPTORS; PLASMA-MEMBRANE; EXTRACELLULAR ATP; HUMAN-LYMPHOCYTES; PHOSPHOLIPASE-D; CA2+ CHANNELS; BAC1.2F5 MACROPHAGES; MICROGLIAL CELLS; P2X(7) RECEPTOR; P-2Z RECEPTOR;
Keywords:
P2X(7) receptor; GH3 cell; intracellular calcium; depolarization; nonselective cation current; membrane permeability;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Lee, JW Yonsei Univ, Wonju Coll Med, Dept Physiol, Wonju 220701, South Korea Yonsei Univ Wonju South Korea 220701 , Wonju 220701, South Korea
Citazione:
H.S. Chung et al., "ATP-induced [Ca2+](i) changes and depolarization in GH3 cells", BR J PHARM, 130(8), 2000, pp. 1843-1852

Abstract

1 Extracellular ATP is a neurotransmitter and mediates a variety of responses. In the endocrine system, there are data suggesting a physiological role for ATP in Ca2+ signalling and hormone secretion. However, the ATP receptor subtype involved has not been clearly elucidated in GH3 cells, a rat anterior pituitary cell line.2 BzATP- and ATP-induced [Ca2+](i) responses had EC50 Values of 18 and 651mu M, respectively. The maximal response to ATP was only 59+/-8% of that for BzATP. The BzATP-induced [Ca2+](i) increase was dependent upon the extracellular Ca2+ concentration. Preincubation with oxidized ATP (oATP) nearly abolished the ATP- and BzATP-induced [Ca2+](i) increases.3 Both BzATP and ATP induced depolarization in GH3 cells, with EC50 valuesof 31 mu M and 1 mM, respectively. The maximal depolarization to BzATP andATP were 152+/-21 and 146+/-16% of that elicited by 30 mM KCI.4 The rank order of agonist potency for [Ca2+](i) and depolarization responses was BzATP > > ATP > 2-MeSATP and purine derivatives such as ADP, AMP, adenosine were ineffective. Neither UTP nor alpha,beta-methylene ATP showedany effect.5 In low-divalent conditions BzATP evoked non-desensitizing inward currents, which were reversed at similar to 0 mV. This nonselective cationic conductance was increased by repeated applications of BzATP and the cells becamevery permeable to NMDG. Longer applications (30 min) of BzATP stimulated ethidium bromide influx in low divalent conditions, suggesting increased permeability to larger molecules. We also identified the existence of P2X(7) mRNA on GH3 cells by using reverse transcriptase (RT)-polymerase chain reaction (PCR).6 These results suggest that the GH3 cells have an endogenous P2X(7) receptor and purinergic stimulation may play a potential role in neuroendocrine modulation on these cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 09:54:49