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Titolo:
Amiodarone interactions with membrane lipids and with growth of Bacillus stearothermophilus used as a model
Autore:
Rosa, SMLJ; Antunes-Madeira, MC; Jurado, AS; Madeira, VVMC;
Indirizzi:
Univ Coimbra, Dept Zool, Ctr Neurociencias, P-3000 Coimbra, Portugal Univ Coimbra Coimbra Portugal P-3000 ociencias, P-3000 Coimbra, Portugal
Titolo Testata:
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
fascicolo: 3, volume: 87, anno: 2000,
pagine: 165 - 175
SICI:
0273-2289(200006)87:3<165:AIWMLA>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENVIRONMENTAL-POLLUTANT DDT; X-RAY-DIFFRACTION; FLUORESCENCE POLARIZATION; BILAYERS; MITOCHONDRIAL; TRANSDUCTION; RESPIRATION; TAMOXIFEN; BACTERIA; FLUIDITY;
Keywords:
amiodarone; Bacillus stearothermophilus; fluorescent probes; membrane organization; bacterial growth;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Madeira, VVMC Univ Coimbra, Dept Zool, Ctr Neurociencias, P-3000 Coimbra, Portugal Univ Coimbra Coimbra Portugal P-3000 000 Coimbra, Portugal
Citazione:
S.M.L.J. Rosa et al., "Amiodarone interactions with membrane lipids and with growth of Bacillus stearothermophilus used as a model", APPL BIOC B, 87(3), 2000, pp. 165-175

Abstract

The thermophilic eubacterium Bacillus stearothermophilus was used as a model to study the effects of amiodarone (2-butyl-3-[3',5'diido-4'alpha-diethyl-aminoethoxybenzoyl]-benzofuran) in lipid organization and in bacterial growth. Effects on the structural order of lipids were assessed by fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene (DPH), probing the bilayercore, and of the propionic acid derivative 3-[p-(6-phenyl)-1,3,5-hexatrienyl] phenylpropionic acid (DPH-PA), probing the outer regions of the bilayer. Amiodarone fluidizes bacterial polar lipid bilayers for temperatures below the phase transition midpoint, and orders the fluid phase of the bacterial polar lipids, as evaluated by DPH and DPH-PA. The ordering and disordering effects, which are concentration dependent, are more extensive when detected by DPH relative to DPH-PA. Growth studies performed in parallel revealed that amiodarone inhibits bacterial growth as a function of concentration. Amiodarone concentrations in the range from 1 to 2.5 mu M increased the lag time, decreased the specific growth rate, and decreased the final cell density. Furthermore, 3 mu M amiodarone completely inhibited growth. These invivo effects of amiodarone can be related to its ability to perturb the phospholipid bilayer structure, whose integrity is essential for cell function, viability, and growth.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 04:53:19