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Titolo:
Monoclonal antibodies to native HIV type 1 reverse transcriptase and theirinteraction with enzymes from different subtypes
Autore:
Rytting, AS; Akerblom, L; Albert, J; Unge, T; Bjorling, E; Al-Khalili, L; Gronowitz, JS; Kallander, CFR;
Indirizzi:
Cavedi Tech AB, SE-75183 Uppsala, Sweden Cavedi Tech AB Uppsala Sweden SE-75183 Tech AB, SE-75183 Uppsala, Sweden Uppsala Univ, BMC, Dept Genet & Pathol, Unit Med Genet, SE-75123 Uppsala, Sweden Uppsala Univ Uppsala Sweden SE-75123 Med Genet, SE-75123 Uppsala, Sweden Natl Vet Inst, Dept Virol, SE-75007 Uppsala, Sweden Natl Vet Inst Uppsala Sweden SE-75007 pt Virol, SE-75007 Uppsala, Sweden Huddinge Univ Hosp, Karolinska Inst, Div Clin Virol, SE-14186 Huddinge, Sweden Huddinge Univ Hosp Huddinge Sweden SE-14186 l, SE-14186 Huddinge, Sweden Karolinska Inst, Ctr Microbiol & Tumor Biol, SE-17177 Stockholm, Sweden Karolinska Inst Stockholm Sweden SE-17177 ol, SE-17177 Stockholm, Sweden Uppsala Univ, BMC, Dept Mol Biol, SE-75123 Uppsala, Sweden Uppsala Univ Uppsala Sweden SE-75123 Mol Biol, SE-75123 Uppsala, Sweden
Titolo Testata:
AIDS RESEARCH AND HUMAN RETROVIRUSES
fascicolo: 13, volume: 16, anno: 2000,
pagine: 1281 - 1294
SICI:
0889-2229(200009)16:13<1281:MATNHT>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; ACTIVITY-BLOCKING ANTIBODY; ANTIGENIC PRESENTATION; DRUG SUSCEPTIBILITY; GAG GENES; GROUP-O; ASSAY; PURIFICATION; POLYMERASE; V3;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Kallander, CFR Cavedi Tech AB, Uppsala Sci Pk, SE-75183 Uppsala, Sweden Cavedi Tech AB Uppsala Sci Pk Uppsala Sweden SE-75183 eden
Citazione:
A.S. Rytting et al., "Monoclonal antibodies to native HIV type 1 reverse transcriptase and theirinteraction with enzymes from different subtypes", AIDS RES H, 16(13), 2000, pp. 1281-1294

Abstract

Recombinant reverse transcriptase (RT) from HIV-1 subtype B was used to produce mouse anti-RT monoclonal antibodies (MAbs). Immunization was done by mixing RT with the ISCOM matrix-forming adjuvant saponin (Quil A). Two different assays, both based on the interaction of native RT and antibodies, were used to monitor the immune response in mice and for screening, selection, and characterization of the MAbs. The first assay measures the capacity of antibodies to inhibit the polymerase activity of the RT and the second assay measures the ability of antibodies to capture enzymatically active RT. Twelve clones with the capacity to inhibit at least 50% of the RT activity and 34 clones with high RT-capturing capacity were found. The MAb panel wasutilized to evaluate the immunological properties of 18 different RTs representing 9 different HIV-1 subtypes. The RT-inhibitory MAbs could be divided into two groups based on their pattern of cross-reactivity toward the different HIV-1 RTs. The degree of diversity recorded among MAbs with RT-capturing capacity was larger. At least seven groups of MAbs with distinct cross-reactivity patterns were identified. Thus, the degree of isoenzyme specificity varied greatly, from MAbs that were quite specific for subtype B RT toone MAb that was able to capture the RTs from all HIV-1 isolates tested except one of the two group O isolates. In conclusion, our study revealed that there exist surprisingly large immunological differences between RTs fromdifferent HIV-1 subtypes as well as from the same subtype.

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Documento generato il 05/12/20 alle ore 16:28:28