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Titolo:
Isolation of Pichia pastoris genes involved in ER-to-Golgi transport
Autore:
Payne, WE; Kaiser, CA; Bevis, BJ; Soderholm, J; Fu, D; Sears, IB; Glick, BS;
Indirizzi:
Univ Chicago, Dept Mol Genet & Cell Biol, Chicago, IL 60637 USA Univ Chicago Chicago IL USA 60637 enet & Cell Biol, Chicago, IL 60637 USA MIT, Dept Biol, Cambridge, MA 02139 USA MIT Cambridge MA USA 02139MIT, Dept Biol, Cambridge, MA 02139 USA
Titolo Testata:
YEAST
fascicolo: 11, volume: 16, anno: 2000,
pagine: 979 - 993
SICI:
0749-503X(200008)16:11<979:IOPPGI>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOPLASMIC-RETICULUM; SACCHAROMYCES-CEREVISIAE; VESICLE FORMATION; SECRETORY PATHWAY; MOLECULAR ANALYSIS; SEQUENCE-ANALYSIS; ESCHERICHIA-COLI; BINDING PROTEIN; HOST STRAINS; YEAST;
Keywords:
secretion; vesicular transport; endoplasmic reticulum; Golgi apparatus; Pichia pastoris; Saccharomyces cerevisiae; introns; splicing;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
70
Recensione:
Indirizzi per estratti:
Indirizzo: Glick, BS Univ Chicago, Dept Mol Genet & Cell Biol, 920 E 58Th St, Chicago, IL 60637USA Univ Chicago 920 E 58Th St Chicago IL USA 60637 ago, IL 60637USA
Citazione:
W.E. Payne et al., "Isolation of Pichia pastoris genes involved in ER-to-Golgi transport", YEAST, 16(11), 2000, pp. 979-993

Abstract

Pichia pastoris has discrete transitional ER sites and coherent Golgi stacks, making this yeast an ideal system for studying the organization of the early secretory pathway, To provide molecular tools for this endeavour, we isolated P, pastel-is homologues of the SEC12, SEC13, SEC17, SEC18 and SAR1genes. The P. pastoris SEC12, SEC13, SEC17 and SEC18 genes were shown to complement the corresponding S. cerevisiae mutants, The SEC17 and SAR1 genescontain introns at the same relative positions in both P. pastoris and S. cerevisiae, whereas the SEC13 gene contains an intron in P. pastoris but not in S, cerevisiae, Intron structure is similar in the two yeasts, althoughthe favoured 5' splice sequence appears to be GTAAGT in P. pastoris vs. GTATGT in S. cerevisiae. The predicted amino acid sequences of Sec13p, Sec17p, Sec18p and Sar1p show strong conservation in the two yeasts. By contrast,the predicted lumenal domain of Sec12p is much larger in P. pastoris, suggesting that this domain may help localize Sec12p to transitional ER sites. A comparison of the SEC12 loci in various budding yeasts indicates that theSEC12-related gene SED4 is probably unique to the Saccharomyces lineage. GenBank Accession Nos are: SEC12, AF216960; SEC13, AF242186; SEC17, AF216957; SEC18, AF216958; SAR1, AF216959; ACT1, AF216956. Copyright (C) 2000 John Wiley & Sons, Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 03:07:33