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Titolo:
Isolation and properties of human alpha-fetoprotein from HepG2 cell cultures
Autore:
Deutsch, HF; Taniguchi, N; Evenson, MA;
Indirizzi:
Univ Wisconsin, Dept Biomol Chem, Madison, WI 53706 USA Univ Wisconsin Madison WI USA 53706 pt Biomol Chem, Madison, WI 53706 USA Univ Wisconsin, Dept Pathol, Madison, WI 53706 USA Univ Wisconsin MadisonWI USA 53706 n, Dept Pathol, Madison, WI 53706 USA Univ Wisconsin, Dept Lab Med, Madison, WI 53706 USA Univ Wisconsin Madison WI USA 53706 , Dept Lab Med, Madison, WI 53706 USA Univ Wisconsin, Dept Med, Madison, WI 53706 USA Univ Wisconsin Madison WIUSA 53706 nsin, Dept Med, Madison, WI 53706 USA Osaka Univ, Sch Med, Dept Biochem, Osaka, Japan Osaka Univ Osaka JapanOsaka Univ, Sch Med, Dept Biochem, Osaka, Japan
Titolo Testata:
TUMOR BIOLOGY
fascicolo: 5, volume: 21, anno: 2000,
pagine: 267 - 277
SICI:
1010-4283(200009/10)21:5<267:IAPOHA>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN LYMPHOCYTES-T; ARACHIDONIC-ACID; TUMOR-CELLS; FATTY-ACIDS; IN-VITRO; RAT RHABDOMYOSARCOMA; HEPATOMA-CELLS; LYMPHOID-CELLS; RECEPTORS; GROWTH;
Keywords:
alpha-fetoprotein; HepG2 cells; fractionation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Deutsch, HF Univ Wisconsin, Dept Biomol Chem, 1300 Univ Ave, Madison, WI 53706 USA Univ Wisconsin 1300 Univ Ave Madison WI USA 53706 WI 53706 USA
Citazione:
H.F. Deutsch et al., "Isolation and properties of human alpha-fetoprotein from HepG2 cell cultures", TUMOR BIOL, 21(5), 2000, pp. 267-277

Abstract

A relatively rapid 3-step fractionation method has been developed for the isolation of human alpha-fetoprotein from culture fluids of HepG2 cells applicable to large volumes. The protein exists as a complex with lipids or lipoproteins but an ethanol precipitation step is effective in separating it. Yields of 50-60% can be obtained from culture fluid containing 30-40 mu g/ml. A minor fraction that appears to be a proteolytic product of the AFP ispresent in the final product. Copyright (C) 2000 S. Karger AG, Basel.

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Documento generato il 16/07/20 alle ore 19:06:34