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Titolo:
Isolation and characterization of a serine protease from the sprouts of Pleioblastus hindsii Nakai
Autore:
Arima, K; Uchikoba, T; Yonezawa, H; Shimada, M; Kaneda, M;
Indirizzi:
Kagoshima Univ, Fac Sci, Dept Chem, Kagoshima 8900065, Japan Kagoshima Univ Kagoshima Japan 8900065 pt Chem, Kagoshima 8900065, Japan
Titolo Testata:
PHYTOCHEMISTRY
fascicolo: 6, volume: 54, anno: 2000,
pagine: 559 - 565
SICI:
0031-9422(200007)54:6<559:IACOAS>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUBSTRATE-SPECIFICITY; TOMATO PLANTS; MELON FRUIT; SUBTILISIN; GENE; SARCOCARP; CUCUMISIN; PURIFICATION; EXPRESSION; SEQUENCE;
Keywords:
Pleioblastus hindsii; Gramineae; bamboo sprout; cucumisin; serine protease; plant protease;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Uchikoba, T Kagoshima Univ, Fac Sci, Dept Chem, Kagoshima 8900065, Japan Kagoshima Univ Kagoshima Japan 8900065 oshima 8900065, Japan
Citazione:
K. Arima et al., "Isolation and characterization of a serine protease from the sprouts of Pleioblastus hindsii Nakai", PHYTOCHEM, 54(6), 2000, pp. 559-565

Abstract

An endopeptidase has been purified from sprouts of bamboo (Pleioblastus hindsii Nakai) to electrophoretic homogeneity by four. purification steps. Its M-r was estimated to be 82 kDa by SDS-PAGE. Enzyme activity was inhibitedstrongly by diisopropyl fluorophosphate, and weakly by p-chloromercuriphenylsulfonic acid, but not at all by EDTA or pepstatin, indicating that it was a serine protease. The preferential cleavage sites for this protease werefound to be large hydrophobic and amide residues at the P-1 position. The specificity of the bamboo serine protease differed from that of cucumisin [EC 3.4.21.25], which cleaved the charged amino acid residues at the P-1 position. (C) 2000 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/09/20 alle ore 02:23:01