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Titolo:
5-Hydroxytryptamine-induced outward currents mediated via 5-HT1A receptorsin neurons of the rat dorsolateral septal nucleus
Autore:
Yamada, K; Hasuo, H; Murakami, C; Yasaka, Y; Fujimura, T; Akasu, T;
Indirizzi:
Kurume Univ, Sch Med, Dept Physiol, Kurume, Fukuoka 8300011, Japan Kurume Univ Kurume Fukuoka Japan 8300011 , Kurume, Fukuoka 8300011, Japan
Titolo Testata:
NEUROSCIENCE RESEARCH
fascicolo: 4, volume: 37, anno: 2000,
pagine: 307 - 314
SICI:
0168-0102(200008)37:4<307:5OCMV5>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
DORSAL RAPHE NEURONS; QUANTITATIVE AUTORADIOGRAPHY; SEROTONIN RECEPTORS; BRAIN; INVITRO; AGONIST; 8-HYDROXY-2-(DI-N-PROPYLAMINO)TETRALIN; ANTAGONISTS; RECORDINGS; 8-OH-DPAT;
Keywords:
lateral septum; serotonin; 5-HT1A receptors; inward rectifier K+ channel; Ba2+; uptake inhibitor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Akasu, T Kurume Univ, Sch Med, Dept Physiol, 67 Asahi Machi, Kurume, Fukuoka 8300011, Japan Kurume Univ 67 Asahi Machi Kurume Fukuoka Japan 8300011 11, Japan
Citazione:
K. Yamada et al., "5-Hydroxytryptamine-induced outward currents mediated via 5-HT1A receptorsin neurons of the rat dorsolateral septal nucleus", NEUROSCI RE, 37(4), 2000, pp. 307-314

Abstract

Effects of 5-hydroxytryptamine (5-HT) on neurons of the rat dorsolateral septal nucleus (DLSN) were examined by intracellular and whole-cell patch-clamp recording techniques. An outward current was induced by 5-HT (1-100 mu M) in a concentration-dependent manner. The EC50 for 5-HT was 4.8 mu M. Also, 8-OH-DPAT (10-100 mu M) produced the outward current an EC50 of 17 mu M. Amplitudes of the outward currents produced by 5-HT (100 mu M) and 8-OH-DPAT (100 mu M) were 117 +/- 4 (n = 6) and 58 +/- 8 pA (n = 6), respectively. Fluvoxamine (200 nM), a specific serotonin re-uptake inhibitor, enhanced the 5-HT (1 mu M) - induced outward current: the EC50 for 5-HT was 0.5 mu M in the presence of fluvoxamine (200 nM). L-694 247 (100 mu M) and CP 93129 (100 mu M) also produced outward currents with amplitudes of 33 +/- 3 (n = 4) and 18 +/- 5 pA (n = 4), respectively in DLSN neurons. DOI (100 mu M) and RS 67333 (100 mu M) did not produce outward currents. NAN 190 shifted, ina parallel manner, the concentration-response relationship of 5-HT to the right. The Lineweaver-Burk plot of the concentration-response curve showed that NAN-190 depressed the 5-HT-induced current in a competitive manner. The current-voltage relationship indicates that the 5-HT-induced current reversed polarity at a potential close to the equilibrium potential of K+. Ba2(100 mu M-1 mM) partially depressed the outward current produced by 5-HT. These results suggest that 5-HT induces multiple K+ currents via 5-HT1A receptors in DLSN neurons. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 00:37:49