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Titolo:
Periodic conformational changes in rRNA: Monitoring the dynamics of translating ribosomes
Autore:
Polacek, N; Patzke, S; Nierhaus, KH; Barta, A;
Indirizzi:
Univ Vienna, Bioctr, Inst Biochem, A-1030 Vienna, Austria Univ Vienna Vienna Austria A-1030 , Inst Biochem, A-1030 Vienna, Austria Max Planck Inst Mol Genet, D-14195 Berlin, Germany Max Planck Inst Mol Genet Berlin Germany D-14195 D-14195 Berlin, Germany
Titolo Testata:
MOLECULAR CELL
fascicolo: 1, volume: 6, anno: 2000,
pagine: 159 - 171
SICI:
1097-2765(200007)6:1<159:PCCIRM>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI RIBOSOME; LEAD-CATALYZED CLEAVAGE; TRANSFER-RNA BINDING; ELONGATION-FACTOR-G; ANGSTROM-RESOLUTION; CRYOELECTRON MICROSCOPY; 70S RIBOSOME; PROTEIN-SYNTHESIS; MESSENGER-RNA; METAL-IONS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Barta, A Univ Vienna, Bioctr, Inst Biochem, Dr Bohr Gasse 9, A-1030 Vienna, Austria Univ Vienna Dr Bohr Gasse 9 Vienna Austria A-1030 ienna, Austria
Citazione:
N. Polacek et al., "Periodic conformational changes in rRNA: Monitoring the dynamics of translating ribosomes", MOL CELL, 6(1), 2000, pp. 159-171

Abstract

In protein synthesis, a tRNA transits the ribosome via consecutive bindingto the A (acceptor), P (peptidyl), and E (exit) site; these tRNA movementsare catalyzed by elongation factor G (EF-G) and GTP. Site-specific Pb2+ cleavage was applied to trace tertiary alterations in tRNA and all rRNAs on pre- and posttranslocational ribosomes. The cleavage pattern of deacylated tRNA and AcPhe-tRNA changed individually upon binding to the ribosome; however, these different conformations were unaffected by translocation. On the other hand, translocation affects 23S rRNA structure. Significantly, the Pb2+ cleavage pattern near the peptidyl transferase center was different before and after translocation. This structural rearrangement emerged periodically during elongation, thus providing evidence for a dynamic and mobile role of 235 rRNA in translocation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 02:37:17