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Titolo:
Abnormal polarization and axon outgrowth in retinal ganglion cells lackingthe POU-domain transcription factor Brn-3b
Autore:
Wang, SW; Gan, L; Martin, SE; Klein, WH;
Indirizzi:
Univ Texas, MD Anderson Canc Ctr, Dept Biochem & Mol Biol, Houston, TX 77030 USA Univ Texas Houston TX USA 77030 Biochem & Mol Biol, Houston, TX 77030 USA
Titolo Testata:
MOLECULAR AND CELLULAR NEUROSCIENCE
fascicolo: 2, volume: 16, anno: 2000,
pagine: 141 - 156
SICI:
1044-7431(200008)16:2<141:APAAOI>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
MICROTUBULE-ASSOCIATED PROTEIN-2; CENTRAL-NERVOUS-SYSTEM; CAENORHABDITIS-ELEGANS; HIPPOCAMPAL-NEURONS; VERTEBRATE RETINA; DIFFERENTIATION; MOUSE; LOCALIZATION; EXPRESSION; BRAIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Klein, WH Univ Texas, MD Anderson Canc Ctr, Dept Biochem & Mol Biol, 1515 Holcombe Blvd, Houston, TX 77030 USA Univ Texas 1515 Holcombe Blvd Houston TX USA 77030 TX 77030 USA
Citazione:
S.W. Wang et al., "Abnormal polarization and axon outgrowth in retinal ganglion cells lackingthe POU-domain transcription factor Brn-3b", MOL CELL NE, 16(2), 2000, pp. 141-156

Abstract

The POU domain transcription factor Brn-3b (also called Brn-3.2) is essential for the normal development of retinal ganglion cells (RGCs) in the mouse. Without Brn-3b, RGCs commit to their fate and migrate to the ganglion cell layer, but most cells die during fetal development. An earlier report (L. can ef al., 1999, Dev. Biol. 210, 469-480) suggested that cell death was caused by abnormal axon formation. Here, we use retinal explants from wild-type and mutant embryos to show that bm-3b-deficient RGCs are not properly polarized and tend to form dendrites rather than axons. Compared with wild-type explants, neurites of RGCs from bm-3b-deficient retinal explants grew slower, were shorter, and did not fasciculate properly. Mutant neurites hadmore microtubules than wildtype controls, and the arrangement of microtubules and neurofilaments was characteristic of dendrites rather than axons. Neurites from individual mutant RGCs displayed abnormal polarity and had dendrite-like branches extending outward from their main axis. Most mutant RGCs exhibited abnormal migratory behavior, and their neurites labeled intensely with the dendrite marker MAP-2. A small number of mutant RGCs were not migratory, and their neurites were longer and labeled positively for the axon marker tau-l, suggesting that some RGCs were not as severely affected by the absence of Brn-3b as others. Although tau-l was not observed in most mutant neurites, it did accumulate in mutant cell bodies, implying that the absence of Brn-3b caused a defect in axon transport. Thus, Brn-3b appears tocontrol the activity of genes that function in establishing RGC polarity, and without Brn-3b, RGCs cannot extend normal axons.

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Documento generato il 23/01/20 alle ore 12:21:30