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Titolo:
Expression of GAP-43 and SCG10 mRNAs in lateral geniculate nucleus of normal and monocularly deprived macaque monkeys
Autore:
Higo, N; Oishi, T; Yamashita, A; Matsuda, K; Hayashi, M;
Indirizzi:
Electrotech Lab, Informat Sci Div, Neurosci Sect, Tsukuba, Ibaraki 3058568, Japan Electrotech Lab Tsukuba Ibaraki Japan 3058568 uba, Ibaraki 3058568, Japan Univ Tsukuba, Sch Med, Dept Physiol, Tsukuba, Ibaraki 3058575, Japan Univ Tsukuba Tsukuba Ibaraki Japan 3058575 sukuba, Ibaraki 3058575, Japan Nihon Univ, Sch Med, Dept Anat, Itabashi Ku, Tokyo 1738610, Japan Nihon Univ Tokyo Japan 1738610 t Anat, Itabashi Ku, Tokyo 1738610, Japan Kyoto Univ, Primate Res Inst, Dept Cellular & Mol Biol, Aichi 4848506, Japan Kyoto Univ Aichi Japan 4848506 Cellular & Mol Biol, Aichi 4848506, Japan
Titolo Testata:
JOURNAL OF NEUROSCIENCE
fascicolo: 16, volume: 20, anno: 2000,
pagine: 6030 - 6038
SICI:
0270-6474(20000815)20:16<6030:EOGASM>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-ASSOCIATED PROTEIN; GLUTAMIC-ACID DECARBOXYLASE; MESSENGER-RNA EXPRESSION; IN-SITU HYBRIDIZATION; VISUAL-CORTEX; GENE-EXPRESSION; CEREBRAL-CORTEX; PERIGENICULATE NUCLEUS; IMMUNOREACTIVE NEURONS; SYNAPTIC PLASTICITY;
Keywords:
GAP-43; SCG10; LGN; macaque monkey; growth-associated protein; koniocellular; GABA; monocular deprivation; in situ hybridization; activity-dependent regulation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
87
Recensione:
Indirizzi per estratti:
Indirizzo: Oishi, T Electrotech Lab, Informat Sci Div, Neurosci Sect, Tsukuba, Ibaraki 3058568, Japan Electrotech Lab Tsukuba Ibaraki Japan 3058568 aki 3058568, Japan
Citazione:
N. Higo et al., "Expression of GAP-43 and SCG10 mRNAs in lateral geniculate nucleus of normal and monocularly deprived macaque monkeys", J NEUROSC, 20(16), 2000, pp. 6030-6038

Abstract

We performed nonradioactive in situ hybridization histochemistry (ISH) in the lateral geniculate nucleus (LGN) of the macaque monkey to investigate the distribution of mRNA for two growth-associated proteins, GAP-43 and SCG10. GAP-43 and SCG10 mRNAs were coexpressed in most neurons of both magnocellular layers (layers I and II) and parvocellular layers (layers III-VI). Double-labeling using nonradioactive ISH and immunofluorescence revealed thatboth GAP-43 and SCG10 mRNAs were coexpressed with the alpha-subunit of type II calcium/calmodulin-dependent protein kinase, indicating that both mRNAs are expressed also in koniocellular neurons in the LGN. We also showed that GABA-immunoreactive neurons in the LGN did not contain GAP-43 and SCG10 mRNAs, indicating that neither GAP-43 nor SCG10 mRNAs were expressed in inhibitory interneurons in the LGN. GABA-immunoreactive neurons in the perigeniculate nucleus, however, contained both GAP-43 and SCG10 mRNAs, indicatingthat both mRNAs were expressed in inhibitory neurons in the perigeniculatenucleus, which project to relay neurons in the LGN. Furthermore, to determine whether the expression of GAP-43 and SCG10 mRNAs is regulated by visualinput, we performed nonradioactive ISH in the LGN and the primary visual area of monkeys deprived of monocular visual input by intraocular injectionsof tetrodotoxin. Both mRNAs were downregulated in the LGN after monocular deprivation for 5 d or longer. From these results, we conclude that both GAP-43 and SCG10 mRNAs are expressed in the excitatory relay neurons of the monkey LGN in an activity-dependent manner.

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Documento generato il 02/07/20 alle ore 22:13:42