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Titolo:
Activation of porcine oocytes using cyclopiazonic acid, an inhibitor of calcium-dependent ATPases
Autore:
Petr, J; Rozinek, J; Jilek, F; Urbankova, D;
Indirizzi:
Res Inst Anim Prod, Dept Reprod Biol, Prague 10401, Czech Republic Res Inst Anim Prod Prague Czech Republic 10401 gue 10401, Czech Republic Czech Tech Univ, Dept Vet Sci, Prague 16521, Czech Republic Czech Tech Univ Prague Czech Republic 16521 Prague 16521, Czech Republic
Titolo Testata:
JOURNAL OF EXPERIMENTAL ZOOLOGY
fascicolo: 4, volume: 287, anno: 2000,
pagine: 304 - 315
SICI:
0022-104X(20000901)287:4<304:AOPOUC>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR; INTRACELLULAR CA2+ STORES; PROTEIN-KINASE-II; MOUSE OOCYTES; BOVINE OOCYTES; CELL-CYCLE; ULTRASTRUCTURAL-LOCALIZATION; SARCOPLASMIC-RETICULUM; CALMODULIN ANTAGONIST; ENDOPLASMIC-RETICULUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
80
Recensione:
Indirizzi per estratti:
Indirizzo: Petr, J Inst Anim Prod, 10 Uhzineves, Prague 10401, Czech Republic Inst Anim Prod 10 Uhzineves Prague Czech Republic 10401 Republic
Citazione:
J. Petr et al., "Activation of porcine oocytes using cyclopiazonic acid, an inhibitor of calcium-dependent ATPases", J EXP ZOOL, 287(4), 2000, pp. 304-315

Abstract

Cyclopiazonic acid (CPA), a potent inhibitor of endogenous calcium-dependent ATPases, is able to induce parthenogenetic activation in pig oocytes matured in vitro. Sixty-four percent of matured pig eggs cultured with 100 nM CPA for 4 hr were activated. A similar activation rate was observed in oocytes treated with thapsigargin, another inhibitor of calcium-dependent ATPases. The parthenogenetic development of CPA-activated eggs did not proceed beyond the 8-cell stage. The blockage of calcium channels by verapamil only slightly decreased the proportion of CPA-activated pig oocytes. This indicates that the release of calcium from intracellular stores is sufficient foroocyte activation and calcium influx from extracellular sources has no significant role. The significant decrease in CPA-activated oocytes (100 nM ofCPA for 4 hr) after a microinjection of heparin indicated that the mobilization of intracellular calcium stores is mediated through inositol trisphosphate receptors. On the other hand, the only slightly depressed activation rate in oocytes microinjected with ruthenium red and procaine indicates that CPA mobilizes a much smaller amount of calcium through the ryanodine receptors. The marked inhibitory effect of ophiobolin A and W7 on the activation of CPA-treated pig oocytes suggests that the calcium signal, as the second messenger, acts downstream through calmodulin. (C) 2000 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 10:23:09