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Titolo:
Rapid activation of gill Na+,K+-ATPase in the euryhaline teleost Fundulus heteroclitus
Autore:
Mancera, JM; McCormick, SD;
Indirizzi:
Univ Cadiz, Fac Ciencias Mar, Dept Biol Anim, E-11510 Puerto Real, Spain Univ Cadiz Puerto Real Spain E-11510 ol Anim, E-11510 Puerto Real, Spain USGS, Conte Anadromous Fish Res Ctr, Biol Resources Div, Turners Falls, MA01376 USA USGS Turners Falls MA USA 01376 Resources Div, Turners Falls, MA01376 USA
Titolo Testata:
JOURNAL OF EXPERIMENTAL ZOOLOGY
fascicolo: 4, volume: 287, anno: 2000,
pagine: 263 - 274
SICI:
0022-104X(20000901)287:4<263:RAOGNI>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
NA+-K+-ATPASE; SALMON SALMO-SALAR; TILAPIA OREOCHROMIS-MOSSAMBICUS; CHLORIDE CELL; SEAWATER ACCLIMATION; ACTINOMYCIN-D; TIME-COURSE; INVITRO STIMULATION; ESTUARINE TELEOST; SEA-WATER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Mancera, JM Univ Cadiz, Fac Ciencias Mar, Dept Biol Anim, E-11510 Puerto Real, Spain Univ Cadiz Puerto Real Spain E-11510 1510 Puerto Real, Spain
Citazione:
J.M. Mancera e S.D. McCormick, "Rapid activation of gill Na+,K+-ATPase in the euryhaline teleost Fundulus heteroclitus", J EXP ZOOL, 287(4), 2000, pp. 263-274

Abstract

The rapid activation of gill Na+,K+-ATPase was analyzed in the mummichog (Fundulus heteroclitus) and Atlantic salmon (Salmo salar) transferred from low salinity (0.1 ppt) to high salinity (25-35 ppt). In parr and presmolt, Salmo salar gill Na+,K+-ATPase activity started to increase 3 days after transfer. Exposure of Fundulus heteroclitus to 35 ppt seawater (SW) induced a rise in gill Na+,K+-ATPase activity 3 hr after transfer. After 12 hr, the values dropped to initial levels but showed a second significant increase 3 days after transfer. The absence of detergent in the enzyme assay resulted in lower values of gill Na+,K+-ATPase, and the rapid increase after transfer to SW was not observed. Na+,K+-ATPase activity of gill filaments in vitrofor 3 hr increased proportionally to the osmolality of the culture medium (600 mosm/kg > 500 mosm/kg > 300 mosm/kg). Osmolality of 800 mosm/kg resulted in lower gill Na+,K+-ATPase activity relative to 600 mosm/kg. Increasingmedium osmolality to 600 mosm/kg with mannitol also increased gill Na+,K+-ATPase. Cycloheximide inhibited the increase in gill Na+,K+-ATPase activityobserved in hyperosmotic medium in a dose-dependent manner (10(-4) M > 10(-5) M > 10(-6) M). Actinomycin D or bumetanide in the culture (doses of 10(-4) M, 10(-5) M, and 10(-6) M) did not affect gill Na+,K+-ATPase. Injectionof fish with actinomycin D prior to gill organ culture, however, preventedthe increase in gill Na+,K+-ATPase activity in hyperosmotic media. The results show a very rapid and transitory increase in gill Na+,K+-ATPase activity in the first hours after the transfer of Fundulus heteroclitus to SW that is dependent on translational and transcriptional processes. (C) 2000 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 03:55:05