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Titolo:
alpha-MSH acetylation in the pituitary gland of the sea bream (Sparus aurata L.) in response to different backgrounds, confinement and air exposure
Autore:
Arends, RJ; Rotllant, J; Metz, JR; Mancera, JM; Bonga, SEW; Flik, G;
Indirizzi:
Catholic Univ Nijmegen, Fac Sci, Dept Anim Physiol, NL-6525 ED Nijmegen, Netherlands Catholic Univ Nijmegen Nijmegen Netherlands NL-6525 ED egen, Netherlands Univ Autonoma Barcelona, Dept Biol Cellular & Fysiol, Unitat Fysiol Anim, Bellaterra, Spain Univ Autonoma Barcelona Bellaterra Spain Fysiol Anim, Bellaterra, Spain Univ Cadiz, Fac Ciencias Mar, Dept Biol Anim, Cadiz 11510, Spain Univ Cadiz Cadiz Spain 11510 ias Mar, Dept Biol Anim, Cadiz 11510, Spain
Titolo Testata:
JOURNAL OF ENDOCRINOLOGY
fascicolo: 2, volume: 166, anno: 2000,
pagine: 427 - 435
SICI:
0022-0795(200008)166:2<427:AAITPG>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
MELANOCYTE-STIMULATING HORMONE; MELANIN-CONCENTRATING HORMONE; TILAPIA OREOCHROMIS-MOSSAMBICUS; PHYSIOLOGICAL COLOR-CHANGE; TROUT ONCORHYNCHUS-MYKISS; CARP CYPRINUS-CARPIO; BETA-ENDORPHIN; XENOPUS-LAEVIS; RAINBOW-TROUT; BRAIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Flik, G Catholic Univ Nijmegen, Fac Sci, Dept Anim Physiol, Toernooiveld 1, NL-6525 ED Nijmegen, Netherlands Catholic Univ Nijmegen Toernooiveld 1 Nijmegen Netherlands NL-6525 ED
Citazione:
R.J. Arends et al., "alpha-MSH acetylation in the pituitary gland of the sea bream (Sparus aurata L.) in response to different backgrounds, confinement and air exposure", J ENDOCR, 166(2), 2000, pp. 427-435

Abstract

MSH is a pituitary hormone derived by post-translational processing from POMC and involved ill stress and background adaptation, N-terminal acetylation of MSH to monoacetyl alpha-MSH or diacetyl alpha-MSH increases the bioactivity of the peptide. The aim of this study was to characterize alpha-MSH acetylation in the sea bream (Sparus aurata L.) pituitary gland in responseto the stressors air exposure and confinement, as well as in fish adapted for 15 days to a white, gray or black background. Pituitary were purified by reversed-phase HPLC (RP-HPLC). The alpha-MSH content of fractions was measured rd by RIA. Immunoreactive RP-HPLC fractions were further analyzed by electrospray mass spectrometry and the peptide sequence determined as SYSMEHFR WGKPV-NH2. In the pituitary gland of sea bream, des-, mono- and diacetyl alpha-MSH were identified. Then plasma alpha-MSH levels were measured in sea bream adapted Cu different backgrounds. Surprisingly, we found the highest plasma alpha-MSH levels in white-adapted as compared with black-adaptedsea bream with intermediate values for gray-adapted fish. This observationis in contrast with results that have been obtained in eel, trout or terrestrial vertebrates. Next, des-, mono- and diacetyl alpha-MSH forms were measured in homogenates of the pituitary gland and in plasma of sea bream exposed to air, to confinement, or to different backgrounds. Monoacetyl alpha-MSH was the predominant form in all control and experimental groups. The lowest content of monoacetyl alpha-MSH relative to des- and diacetyl alpha-MSHwas found in white-adapted fish. Levels of des- and diacetyl alpha-MSH forms were similar under all conditions. We observed that monoacetyl alpha-MSHis the most abundant isoform in the pituitary gland after background adaptation, confinement and air exposure, in sea bream. These data indicate thatthe physiologically most potent isoform of alpha-MSH may vary from speciesto species.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 04:04:25