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Titolo:
Stereoselective quantification of methadone and its major oxidative metabolite, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine, in human urine using high-performance liquid chromatography
Autore:
Foster, DJR; Somogyi, AA; Bochner, F;
Indirizzi:
Univ Adelaide, Dept Clin & Expt Pharmacol, Adelaide, SA 5005, Australia Univ Adelaide Adelaide SA Australia 5005 ol, Adelaide, SA 5005, Australia Royal Adelaide Hosp, Dept Clin Pharmacol, Adelaide, SA 5000, Australia Royal Adelaide Hosp Adelaide SA Australia 5000 elaide, SA 5000, Australia
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1, volume: 744, anno: 2000,
pagine: 165 - 176
SICI:
1387-2273(20000707)744:1<165:SQOMAI>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN LIVER-MICROSOMES; MAIN METABOLITE; N-DEMETHYLATION; CYTOCHROME-P450 3A4; MASS-SPECTROMETRY; MAINTENANCE; INVOLVEMENT; ENANTIOMERS; CAPILLARY; SERUM;
Keywords:
stereoselectivity; methadone; 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Foster, DJR Univ Adelaide, Dept Clin & Expt Pharmacol, Adelaide, SA 5005, Australia Univ Adelaide Adelaide SA Australia 5005 , SA 5005, Australia
Citazione:
D.J.R. Foster et al., "Stereoselective quantification of methadone and its major oxidative metabolite, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine, in human urine using high-performance liquid chromatography", J CHROMAT B, 744(1), 2000, pp. 165-176

Abstract

A stereoselective HPLC assay was developed for the quantification of the enantiomers of methadone and its major oxidative metabolite, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) in human urine. The compounds were quantified in a single assay following liquid-liquid extraction and stereoselective HPLC with UV detection. Calibration curve concentrations ranged from 0.125 to 12.5 mu M for each enantiomer. Assay performance was assessedusing quality control samples, and the inter- and intra-assay bias (<10%) and precision (<15%) were acceptable for all compounds. The assay was successfully used to quantitate the enantiomers of methadone and EDDP in urine samples obtained from subjects receiving methadone maintenance therapy. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 03:45:19