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Titolo:
Effect of murine thymic epithelial cell line (MTEC1) on the functional expression of CD4(+)CD8(-) thymocyte subgroups
Autore:
Ge, Q; Chen, WF;
Indirizzi:
Peking Univ, Hlth Sci Ctr, Dept Immunol, Beijing 100083, Peoples R China Peking Univ Beijing Peoples R China 100083 ijing 100083, Peoples R China
Titolo Testata:
INTERNATIONAL IMMUNOLOGY
fascicolo: 8, volume: 12, anno: 2000,
pagine: 1127 - 1133
SICI:
0953-8178(200008)12:8<1127:EOMTEC>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
T-CELLS; LYMPHOKINE SECRETION; NEGATIVE SELECTION; MATURATION; HELPER; SUBSETS; CD4+; MICE; ACQUISITION; PATTERNS;
Keywords:
CD4(+)CD8(-) thymocyte subgroup; cytokine; proliferation; thymic stromal cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Chen, WF Peking Univ, Hlth Sci Ctr, Dept Immunol, Beijing 100083, Peoples R China Peking Univ Beijing Peoples R China 100083 083, Peoples R China
Citazione:
Q. Ge e W.F. Chen, "Effect of murine thymic epithelial cell line (MTEC1) on the functional expression of CD4(+)CD8(-) thymocyte subgroups", INT IMMUNOL, 12(8), 2000, pp. 1127-1133

Abstract

To determine the effect of thymic stromal cells on the functional maturation of CD4 single-positive (SP) thymocytes, the functional status of isolated CD4 SP thymocyte subgroups was investigated by means of cell proliferation and cytokine production in response to concanavalin A (Con A) prior and after co-culturing with a murine thymic epithelial cell line (MTEC1). Mouse medullary CD4 SP thymocytes were phenotypically divided into seven discretesubgroups predicted to reflect the maturation pathway from newly emerging CD4 SP thymocytes to terminally differentiated cells. For functional analysis, six major subgroups (6C10(+)CD69(+), 6C10(-)CD69(+), 6C10(-)CD69(-)3G11(+)Qa-2(-), 6C10(-)CD69(-)3G11(+)Qa-2(+), 6C10(-)CD69(-)3G11(-)Qa-2(-) and 6C10(-)CD69(-)3G11(-)Qa-(2+)) cells were isolated and their functional status in response to Con A stimulation assessed. A functional hierarchy is revealed among these subgroups, consistent with their phenotypic maturation status, which may imply that these cells undergo a functional maturation process within thymic medulla, The function of cytokine production by CD4 SP thymocytes is acquired in a stepwise manner from a low to high level and characterized by T(h)0-type cytokines in the main stream of differentiation pathway. However, a minor subgroup that appeared at the late stage as 3G11-6C10(-) cells was biased to produce T(h)2-type cytokines. Nevertheless, the functional capacity of the final two Qa-2(+) subgroups of CD4 SP thymocytes was still significantly lower than that of spleen CD4(+) T cells. After co-cultivation with MTEC1 cells, four subgroups of TCR alpha beta(+)CD4(+)CD8(-) thymocytes exhibited significantly higher levels of proliferation capability and modulation in cytokine production capability. However, co-culturingwith MTEC1 cells did not change the pattern of T(h)0- or T(h)2-like cytokine production by respectively medullary CD4 SP thymocyte subgroups nor could MTEC1 induce CD4 SP thymocytes to secrete T(h)1-type cytokines, The results suggest that MTEC1 can regulate the functional status of these thymocytesubgroups.

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Documento generato il 04/04/20 alle ore 21:42:56