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Titolo:
zeta-Crystallin catalyzes the reductive activation of 2,4,6-trinitrotoluene to generate reactive oxygen species: a proposed mechanism for the induction of cataracts
Autore:
Kumagai, Y; Wakayama, T; Li, S; Shinohara, A; Iwamatsu, A; Sun, G; Shimojo, N;
Indirizzi:
Univ Tsukuba, Dept Environm Med, Inst Community Med, Masters Program Environm Sci, Tsukuba, Ibaraki 3058575, Japan Univ Tsukuba Tsukuba Ibaraki Japan 3058575 sukuba, Ibaraki 3058575, Japan Univ Tsukuba, Doctoral Progra Med Sci, Tsukuba, Ibaraki 3058575, Japan Univ Tsukuba Tsukuba Ibaraki Japan 3058575 sukuba, Ibaraki 3058575, Japan China Med Univ, Sch Publ Hlth, Dept Labor Hyg & Occupat Hlth, Shengyang 110001, Peoples R China China Med Univ Shengyang Peoples R China 110001 110001, Peoples R China Kirin Brewery Co Ltd, Cent Labs Key Technol, Kanazawa Ku, Yokohama, Kanagawa 236, Japan Kirin Brewery Co Ltd Yokohama Kanagawa Japan 236 ama, Kanagawa 236, Japan
Titolo Testata:
FEBS LETTERS
fascicolo: 3, volume: 478, anno: 2000,
pagine: 295 - 298
SICI:
0014-5793(20000804)478:3<295:ZCTRAO>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
GUINEA-PIG LENS; SUPEROXIDE-DISMUTASE; LIPID-PEROXIDATION; OXIDATIVE STRESS; BOVINE LENS; RAT LENSES; PROTEINS; TRINITROTOLUENE; PURIFICATION; OXIDOREDUCTASE;
Keywords:
2,4,6-trinitrotoluene; zeta-crystallin; active oxygen; cataract; bovine; lens;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Kumagai, Y Univ Tsukuba, Dept Environm Med, Inst Community Med, Masters Program Environm Sci, Tsukuba, Ibaraki 3058575, Japan Univ Tsukuba Tsukuba Ibaraki Japan 3058575 raki 3058575, Japan
Citazione:
Y. Kumagai et al., "zeta-Crystallin catalyzes the reductive activation of 2,4,6-trinitrotoluene to generate reactive oxygen species: a proposed mechanism for the induction of cataracts", FEBS LETTER, 478(3), 2000, pp. 295-298

Abstract

Exposure to 2,4,6-trinitrotoluene (TNT) has been shown to cause induction of cataract in which oxidative stress plays a critical role, From bovine lens we purified to homogeneity and identified an enzyme that catalyzes the reduction of TNT, resulting in the production of reactive oxygen species. The final preparation of TNT reductase showed a single band with a subunit molecular weight of 38 kDa on SDS-PAGE, Sequence data from peptides obtained by digestion with lysylendopeptidase Achromobacter protease I(API) revealedthat TNT reductase is identical to zeta-crystallin. Superoxide anions wereformed during reduction of TNT by zeta-crystallin, though negligible enzyme activity or protein content for superoxide dismutase, a superoxide scavenging enzyme, was found in the lens. Thus, the present results suggest that the induction of cataracts by TNT may be associated with increased oxidative stress, as a result of reductive activation of TNT generating superoxide anions, there being minimal antioxidant enzyme activity for defense againstreactive oxygen species exogenously produced in the lens. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 10:17:20