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Titolo:
Tracer methodology in whole body and organ balance metabolic studies: plasma sampling is required - A study in post-absorptive rats using isotopically labeled arginine, phenylalanine, valine and leucine
Autore:
Hallemeesch, MM; Soeters, PB; Deutz, NEP;
Indirizzi:
Univ Limburg, Fac 2, Dept Surg, NL-6200 MD Maastricht, Netherlands Univ Limburg Maastricht Netherlands NL-6200 MD D Maastricht, Netherlands
Titolo Testata:
CLINICAL NUTRITION
fascicolo: 3, volume: 19, anno: 2000,
pagine: 157 - 163
SICI:
0261-5614(200006)19:3<157:TMIWBA>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMINO-ACID-TRANSPORT; TUMOR-BEARING RATS; FASTED RATS; HUMAN-ERYTHROCYTES; PROTEIN-TURNOVER; SYSTEM-L; KINETICS; GUT; MUSCLE; BLOOD;
Keywords:
methodology; tracer technology; whole blood; plasma;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Hallemeesch, MM Univ Limburg, Fac 2, Dept Surg, POB 616, NL-6200 MD Maastricht, Netherlands Univ Limburg POB 616 Maastricht Netherlands NL-6200 MD ds
Citazione:
M.M. Hallemeesch et al., "Tracer methodology in whole body and organ balance metabolic studies: plasma sampling is required - A study in post-absorptive rats using isotopically labeled arginine, phenylalanine, valine and leucine", CLIN NUTR, 19(3), 2000, pp. 157-163

Abstract

Background and aims: Radioactive and stable amino acid isotopes are frequently used in metabolic research. Blood cells contain amino acid transporters, which may influence tracer distribution in blood. The aim of this study was to determine whether plasma or whole blood specific activity or enrichment of amino acid tracers should be used in the calculation of whole body and organ production rates. Methods: Seven male Wistar rats were infused with L-[2,3-H-3]-Arginine, L-[2,6-H-3]-Phenylalanine, L-[3,4-H-3]-Valine, and [L-[4,5-H-3]-Leucine, Whole body and portal drained visceral, hepatic and renal production rates of arginine, phenylalanine, valine and leucine were determined in plasma and inwhole blood. Results: Amino acid tracers that equilibrate well between plasma and bloodcells (for instance phenylalanine, valine and leucine) yield similar wholebody production rates when whole blood or plasma is sampled. Also, organ production rates measured using these amino acid tracers are consistent. However, a discrepancy exists between the whole body production rate and the sum of PDV, hepatic and renal production rates. When tracers are used that do not equilibrate well between plasma and blood cells (for instance arginine) the use of whole blood specific activity in the calculations yield overestimations of whole body and organ production rates. Conclusion: From our data we recommend plasma sampling and strongly adviseagainst whole blood sampling in metabolic organ balance studies in which amino acid tracers are used. (C) 2000 Harcourt Publishers Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/21 alle ore 08:03:53