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Titolo:
The clinical usefulness of the measurement of cytokines
Autore:
Bienvenu, J; Monneret, G; Fabien, N; Revillard, JP;
Indirizzi:
Ctr Hosp Lyon Sud, Serv Immunol, F-69495 Pierre Benite, France Ctr Hosp Lyon Sud Pierre Benite France F-69495 495 Pierre Benite, France Jeune Equipe Univ Pathol Cellules Lymphoides, Ctr Hosp Lyon Sud, Pierre Benite, France Jeune Equipe Univ Pathol Cellules Lymphoides Pierre Benite France rance Hop Edouard Herriot, Unite Immunopharmacol, Lyon, France Hop Edouard Herriot Lyon France ot, Unite Immunopharmacol, Lyon, France
Titolo Testata:
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
fascicolo: 4, volume: 38, anno: 2000,
pagine: 267 - 285
SICI:
1434-6621(200004)38:4<267:TCUOTM>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; C-REACTIVE PROTEIN; INFLAMMATORY RESPONSE SYNDROME; DEPENDENT DIABETES-MELLITUS; EPITHELIAL OVARIAN-CANCER; INTERFERON-GAMMA GENE; COMMERCIAL ELISA-KITS; EOTAXIN RECEPTOR CCR3; TNF-ALPHA PRODUCTION; MYELOMA-CELL-GROWTH;
Keywords:
cytokines; cytokine receptors; bioassays; immunoassays; clinical applications;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
152
Recensione:
Indirizzi per estratti:
Indirizzo: Bienvenu, J Ctr Hosp Lyon Sud, Serv Immunol, F-69495 Pierre Benite, FranceCtr Hosp Lyon Sud Pierre Benite France F-69495 enite, France
Citazione:
J. Bienvenu et al., "The clinical usefulness of the measurement of cytokines", CLIN CH L M, 38(4), 2000, pp. 267-285

Abstract

The utilization of accurate and sensitive methods for the measurement of cytokines in body fluids is prerequisite for the proper use of these mediators in clinical practice. Many factors contribute to the complexity of cytokine quantitation. Bioassays historically preceded immunoassays, which are now very popular, but there is a need for standardization. Nevertheless, dueto the local effects of cytokines, the study of their blood levels is of limited value for an understanding of the pathophysiology of these mediators. This explains the development of alternative approaches to assess the ability of cells to produce cytokines. These include the Enzyme-Linked Immune Spot Assay (ELISPOT), the measurement of cell-associated cytokines by flow cytometry, and the study of cytokine secretion by isolated peripheral bloodmononuclear cells or by whole blood test. All these techniques, associatedwith a local detection of cytokines by immunohistochemistry or in situ hybridization and reverse transcriptase polymerase chain reaction, appear to be complementary tools for a better understanding of the biology of cytokines. Selected examples of possible clinical applications related to infectious diseases, cancer, autoimmune diseases, allergy, transplantation and preclinical evaluation of drugs and biotechnology products are given.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 05:26:56