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Titolo:
An IgM lambda antibody to Escherichia coli produces false-positive resultsin multiple immunometric assays
Autore:
Covinsky, M; Laterza, O; Pfeifer, JD; Farkas-Szallasi, T; Scott, MG;
Indirizzi:
Washington Univ, Sch Med, Dept Pathol & Immunol, Div Lab Med, St Louis, MO63110 USA Washington Univ St Louis MO USA 63110 Div Lab Med, St Louis, MO63110 USA Washington Univ, Sch Med, Dept Pathol & Immunol, Div Surg Pathol, St Louis, MO 63110 USA Washington Univ St Louis MO USA 63110 Surg Pathol, St Louis, MO 63110 USA
Titolo Testata:
CLINICAL CHEMISTRY
fascicolo: 8, volume: 46, anno: 2000,
parte:, 1
pagine: 1157 - 1161
SICI:
0009-9147(200008)46:8<1157:AILATE>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
CARDIAC TROPONIN-I; CREATINE-KINASE-MB; MOUSE ANTIBODIES; HETEROPHILIC ANTIBODIES; CLONAL CHARACTERIZATION; B POLYSACCHARIDE; INTERFERENCE; REPERTOIRE; SPECIFICITY; RESTRICTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Scott, MG Washington Univ, Sch Med, Dept Pathol & Immunol, Div Lab Med, StLouis, MO63110 USA Washington Univ St Louis MO USA 63110 ed, St Louis, MO63110 USA
Citazione:
M. Covinsky et al., "An IgM lambda antibody to Escherichia coli produces false-positive resultsin multiple immunometric assays", CLIN CHEM, 46(8), 2000, pp. 1157-1161

Abstract

Background: Interferences in immunometric assays as a result of human anti-immunoglobulin antibodies frequently have been described in the literature. The etiology of these interfering antibodies is usually not known but hasbeen associated with rheumatoid factors in some assays. It is known that microorganisms in experimental settings can induce anti-immunoglobulin antibodies. Methods: Following Escherichia coli septicemia, a 56-year-old male patienthad increased immunoassay results for cardiac troponin I, thyrotropin, human chorionic gonadotropin, alpha-fetoprotein, and CA-125 that were consistent with myocardial infarction, hyperthyroidism, and pregnancy, and suggestive of an occult neoplasm such as hepatic or ovarian cancer. None of these diagnoses were consistent with the rest of his medical exam. In addition, the patient had a restricted IgM lambda paraprotein by immunofixation. Plasmafrom the patient was incubated with Sepharose-conjugated protein A, irrelevant murine monoclonal antibodies, and formalin-killed E. coli organisms from his infection to determine whether these immunoassay values were falselyincreased. Results: Incubation of the patient's plasma with irrelevant murine monoclonal antibodies or the E. coli organism produced normal immunoassay values and removed the IgM lambda paraprotein. Conclusions: The patient produced a very restricted IgM lambda antibody response to the E. coli infection that had anti-immunoglobulin activity and caused falsely increased values in numerous immunometric assays. Microorganism-induced anti-immunoglobulin antibodies are discussed in the context of this patient. (C) 2000 American Association for Clinical Chemistry.

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Documento generato il 20/09/20 alle ore 00:31:19