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Titolo:
Expression of cytochrome P450 1A1/2 and 3A4 in liver tissues of hepatocellular carcinoma cases and controls from Taiwan and their relationship to hepatitis B virus and aflatoxin B-1- and 4-aminobiphenyl-DNA adducts
Autore:
Zhang, YJ; Chen, SY; Tsai, WY; Ahsan, H; Lunn, RM; Wang, LY; Chen, CJ; Santella, RM;
Indirizzi:
Columbia Univ, Mailman Sch Publ Hlth, Div Environm Hlth Sci, New York, NY 10032 USA Columbia Univ New York NY USA 10032 ronm Hlth Sci, New York, NY 10032 USA Columbia Univ, Mailman Sch Publ Hlth, Div Biostat, New York, NY 10032 USA Columbia Univ New York NY USA 10032 , Div Biostat, New York, NY 10032 USA
Titolo Testata:
BIOMARKERS
fascicolo: 4, volume: 5, anno: 2000,
pagine: 295 - 306
SICI:
1354-750X(200007/08)5:4<295:EOCP1A>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSGENIC MICE; MESSENGER-RNA; ENZYMES; ACTIVATION; METABOLISM; INDUCTION; EXPOSURE; LOCALIZATION; CARRIERS; PROTEIN;
Keywords:
carcinogen-DNA adducts; cigarette smoking; immunohistochemistry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Santella, RM Columbia Univ, Mailman Sch Publ Hlth, Div Environm Hlth Sci, 701 W 168th St, New York, NY 10032 USA Columbia Univ 701 W 168th St New York NY USA 10032 10032 USA
Citazione:
Y.J. Zhang et al., "Expression of cytochrome P450 1A1/2 and 3A4 in liver tissues of hepatocellular carcinoma cases and controls from Taiwan and their relationship to hepatitis B virus and aflatoxin B-1- and 4-aminobiphenyl-DNA adducts", BIOMARKERS, 5(4), 2000, pp. 295-306

Abstract

Cytochrome P450 enzymes play a major role in the metabolism of several of the chemical carcinogens involved in the development of hepatocellular carcinoma (HCC). To investigate by immunohistochemistry interindividual differences in these enzymes, polyclonal antisera and immunoperoxidase staining were used to detect the expression of CYP1A1/2 and 3A4 in 37 surgical controltissues and 105 tumour and adjacent non-tumour tissues of HCC cases from Taiwan. There was variability in the expression and staining pattern for both CYP1A1/2 and 3A4 in all tissue types. In tissues from controls, there wasno correlation between P450 expression and smoking history or hepatitis B virus antigen status. Since these samples had been previously analysed for the DNA adducts of aflatoxin B-1 (AFB(1)), a dietary mould contaminant, and4-aminobiphenyl (4-ABP), a component of cigarette smoke, we also investigated the relationship between P450 levels and DNA adducts. 4-ABP-DNA adductswere higher in tissues with elevated levels of CYP1A1/2 (p = 0.02). Overall there was no relationship between CYP1A1/2 or CYP3A4 and AFB(1)-DNA adducts in control tissues. Staining intensity for CYP1A1/2 and 3A4 followed theorder: tumour tissues <control tissues <adjacent non-tumour tissues. CYP1A1/2 levels tended to be lower in tumour and adjacent non-tumour tissues than for CYP3A4. In HCC cases, 4-ABP-DNA adducts were higher in subjects with higher levels of CYP1A1/2, stratified by tissue type, but these differenceswere not significant. For CYP3A4, in contrast to control tissues, there was a significant association with AFB(1)-DNA adducts in tumour and adjacent non-tumour tissue of HCC cases. These results suggest that one factor influencing carcinogen-DNA adducts is levels of specific P450 enzymes. However, adduct formation in vivo is a complex processes dependent upon numerous genetic and environmental factors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 01:06:22