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Titolo:
Endothelin-1 is a potent activator of nonselective cation currents in human bronchial smooth muscle cells
Autore:
Oonuma, H; Nakajima, T; Nagata, T; Iwasawa, K; Wang, YP; Hazama, H; Morita, Y; Wang, Y; Yamamoto, K; Nagai, R; Omata, M;
Indirizzi:
Univ Tokyo, Grad Sch Med, Dept Cardiovasc Med, Bunkyo Ku, Tokyo 1138655, Japan Univ Tokyo Tokyo Japan 1138655 vasc Med, Bunkyo Ku, Tokyo 1138655, Japan Univ Tokyo, Grad Sch Med, Dept Resp Med, Bunkyo Ku, Tokyo 1138655, Japan Univ Tokyo Tokyo Japan 1138655 Resp Med, Bunkyo Ku, Tokyo 1138655, Japan Univ Tokyo, Grad Sch Med, Dept Gastroenterol, Bunkyo Ku, Tokyo 1138655, Japan Univ Tokyo Tokyo Japan 1138655 oenterol, Bunkyo Ku, Tokyo 1138655, Japan
Titolo Testata:
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
fascicolo: 2, volume: 23, anno: 2000,
pagine: 213 - 221
SICI:
1044-1549(200008)23:2<213:EIAPAO>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
PIG TRACHEAL MYOCYTES; PATCH-CLAMP TECHNIQUES; EPITHELIAL-CELLS; VASOCONSTRICTOR PEPTIDE; CA2+ CURRENT; CANINE; EXPRESSION; CHANNELS; INFLUX; MECHANISMS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Nakajima, T Univ Tokyo, Grad Sch Med, Dept Cardiovasc Med, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1138655, Japan Univ Tokyo 7-3-1 Hongo Tokyo Japan 1138655 kyo 1138655, Japan
Citazione:
H. Oonuma et al., "Endothelin-1 is a potent activator of nonselective cation currents in human bronchial smooth muscle cells", AM J RESP C, 23(2), 2000, pp. 213-221

Abstract

The effects of endothelin (ET)-1 on cultured human bronchial smooth musclecells (HBSMC) were investigated and compared with those of histamine, using the patch clamp techniques and measurements of intracellular Ca2+ ([Ca2+](i)). Both ET-1 and histamine caused an initial transient elevation of [Ca2](i) by Ca2+ mobilization, followed by a sustained rise due to Ca2+ entry. Nicardipine inhibited the sustained phase, but La3+ abolished it. With lowethyleneglycol-bis-(beta-aminoethyl ether)-N,N'-tetraacetic acid (EGTA) and K+ internal solutions, both ET-1 and histamine induced a sustained depolarization from approximately -40 to -20 mV. Under voltage clamp conditions, both drugs transiently activated an outward K+ current at a holding potential of 0 mV. Additionally, with a Cs' internal solution, they elicited another transient inward current, frequently followed by current oscillations. These transient currents were blocked by high EGTA or heparin. With high EGTA and Cs+ Internal solutions, both drugs activated a long-lasting inward current. The reversal potential of these agonist-induced currents was approximately 0 mV and was not altered by the replacement of internal or external concentration of CI, suggesting that the inward current was a nonselective cation current (I-cat). The half-maximal effective concentration to activate I-cat was 12 nM for ET-1 and 11 mu M for histamine. La3+ and Cd2+ abolished these agonist-induced I-cat. The effects of ET-1 on [Ca2+](i) and I-cat could be blocked by combined pretreatment with BQ-123 and BQ-788. Sarafotoxin S6c also increased [Ca2+](i) and activated I-cat. By polymerase chain reaction of reverse transcribed RNA, we detected both ET-A and ET-B receptor messenger RNA. These results provide the first evidence that ET-1 is a potent activator of I-cat in HBSMC via ET-A and ET-B receptors, and the activation of I-cat plays an important role in ET-l-induced Ca2+ entry in human airways.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 13:30:53