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Titolo:
Stress causes decrease in vascular relaxation linked with altered phosphorylation of heat shock proteins
Autore:
Fuchs, LC; Giulumian, AD; Knoepp, L; Pipkin, W; Dickinson, M; Hayles, C; Brophy, C;
Indirizzi:
Med Coll Georgia, Vasc Biol Ctr, Augusta, GA 30912 USA Med Coll Georgia Augusta GA USA 30912 asc Biol Ctr, Augusta, GA 30912 USA Med Coll Georgia, Dept Pharmacol & Toxicol, Augusta, GA 30912 USA Med CollGeorgia Augusta GA USA 30912 ol & Toxicol, Augusta, GA 30912 USA Med Coll Georgia, Dept Surg, Augusta, GA 30912 USA Med Coll Georgia Augusta GA USA 30912 a, Dept Surg, Augusta, GA 30912 USA Med Coll Georgia, Inst Mol Med & genet, Dept Med, Augusta, GA 30912 USA Med Coll Georgia Augusta GA USA 30912 et, Dept Med, Augusta, GA 30912 USA Med Coll Georgia, Dept Anat & Cell Biol, Augusta, GA 30912 USA Med Coll Georgia Augusta GA USA 30912 & Cell Biol, Augusta, GA 30912 USA Vet Adm Med Ctr, Augusta, GA 30904 USA Vet Adm Med Ctr Augusta GA USA 30904 t Adm Med Ctr, Augusta, GA 30904 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY
fascicolo: 2, volume: 279, anno: 2000,
pagine: R492 - R498
SICI:
0363-6119(200008)279:2<R492:SCDIVR>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
SMOOTH-MUSCLE CONTRACTION; BORDERLINE HYPERTENSIVE RAT; UMBILICAL ARTERY; NITRIC-OXIDE; EXPRESSION; DISEASE; HSP20; VASORELAXATION; INCREASES; KINASE;
Keywords:
behavioral stress; vascular smooth muscle; hypertension;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Fuchs, LC Med Coll Georgia, Vasc Biol Ctr, Augusta, GA 30912 USA Med Coll Georgia Augusta GA USA 30912 tr, Augusta, GA 30912 USA
Citazione:
L.C. Fuchs et al., "Stress causes decrease in vascular relaxation linked with altered phosphorylation of heat shock proteins", AM J P-REG, 279(2), 2000, pp. R492-R498

Abstract

Cyclic nucleotide-dependent vascular relaxation is associated with increases in the phosphorylation of a small heat shock protein (HSP), HSP20. An increase in phosphorylation of another small HSP, HSP27, is associated with impaired cyclic nucleotide-dependent vascular relaxation. Expression of HSPsis altered by exposure to several types of cellular stress in vitro. To determine if behavioral stress in vivo alters vascular expression and phosphorylation of the small HSPs and cyclic nucleotide-dependent vascular relaxation, borderline hypertensive rats were stressed by restraint and exposure to air-jet stress 2 h/day for 10 days or remained in their home cage. Stressimpaired relaxation of aorta to forskolin, which activates adenylyl cyclase, and sodium nitroprusside, which activates guanylyl cyclase. This was associated with an increase in the aortic expression and phosphorylation of HSP27, which was localized to the vascular smooth muscle, but a decrease in the amount of phosphorylated (P)-HSP20. To determine if P-HSP27 inhibits phosphorylation of HSP20, P-HSP27 was added to a reaction mixture containing recombinant HSP20 and the catalytic subunit of cAMP-dependent protein kinase. P-HSP27 inhibited phosphorylation of HSP20 in a concentration-dependent manner. These data demonstrate that P-HSP27 can inhibit phosphorylation of HSP20. The increase in P-HSP27 and decrease in P-HSP20 were associated with reduced cyclic nucleotide-dependent vascular smooth muscle relaxation in response to behavioral stress in vivo, an effect similar to that observed previously in response to cellular stress in vitro.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 10:57:33