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Titolo:
Differences in regulation of pH(i) in large (>= 10 nuclei) and small (<= 5nuclei) osteoclasts
Autore:
Lees, RL; Heersche, JNM;
Indirizzi:
Univ Toronto, Fac Dent, Toronto, ON M5G 1G6, Canada Univ Toronto Toronto ON Canada M5G 1G6 Dent, Toronto, ON M5G 1G6, Canada Univ Toronto, Fac Med, Dept Pharmacol, Toronto, ON M5G 1G6, Canada Univ Toronto Toronto ON Canada M5G 1G6 macol, Toronto, ON M5G 1G6, Canada
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
fascicolo: 3, volume: 279, anno: 2000,
pagine: C751 - C761
SICI:
0363-6143(200009)279:3<C751:DIROPI>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
MULTINUCLEATED GIANT-CELLS; BONE-RESORPTION; INTRACELLULAR PH; RABBIT OSTEOCLASTS; H+-ATPASE; CYTOPLASMIC PH; INDIVIDUAL OSTEOCLASTS; NA+/H+ ANTIPORTER; AVIAN OSTEOCLAST; CHLORIDE CHANNEL;
Keywords:
vacuolar-type hydrogen-adenosinetriphosphatase; sodium/hydrogen exchanger; nuclear number; intracellular pH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
60
Recensione:
Indirizzi per estratti:
Indirizzo: Heersche, JNM Univ Toronto, Fac Dent, 124 Edward St,Rm 403, Toronto, ON M5G 1G6, Canada Univ Toronto 124 Edward St,Rm 403 Toronto ON Canada M5G 1G6
Citazione:
R.L. Lees e J.N.M. Heersche, "Differences in regulation of pH(i) in large (>= 10 nuclei) and small (<= 5nuclei) osteoclasts", AM J P-CELL, 279(3), 2000, pp. C751-C761

Abstract

Osteoclasts are multinucleated cells that resorb bone by extrusion of protons and proteolytic enzymes. They display marked heterogeneity in cell size, shape, and resorptive activity. Because high resorptive activity in vivo is associated with an increase in the average size of osteoclasts in areas of greater resorption and because of the importance of proton extrusion in resorption, we investigated whether the activity of the bafilomycin A(1)-sensitive vacuolar-type H+-ATPase (V-ATPase) and amiloride-sensitive Na+/H+ exchanger differed between large and small osteoclasts. Osteoclasts were obtained from newborn rabbit bones, cultured on glass coverslips, and loaded with the pH-sensitive indicator 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF). Intracellular pH (pH(i)) was recorded in single osteoclasts by monitoring fluorescence. Large (greater than or equal to 10 nuclei) and small (less than or equal to 5 nuclei) osteoclasts differed in that large osteoclasts had a higher basal pH(i), their pH(i) was decreased by bafilomycin A(1) addition or removal of extracellular Na+, and the realkalinization upon readdition of Na+ was bafilomycin A(1) sensitive. After acid loading, a subpopulation of large osteoclasts (40%) recovered by V-ATPase activity alone, whereas all small osteoclasts recovered by Na+/H+ exchanger activity. Interestingly, in 60% of the large osteoclasts, pH(i) recovery was mediated by both the Na+/H+ exchanger and V-ATPase activity. Our results show a striking difference between pH(i) regulatory mechanisms of large and small osteoclasts that we hypothesize may be associated with differences in the potential resorptive activity of these cells.

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Documento generato il 02/04/20 alle ore 04:30:54