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Titolo:
SANS MEASUREMENTS ON SULFOLOBUS-SOLFATARICUS RIBOSOME AS A FUNCTION OF TEMPERATURE AND MAGNESIUM CONCENTRATION
Autore:
BRIGANTI G; GIORDANO R; LONDEI P; PEDONE F;
Indirizzi:
UNIV MESSINA,DEPT PHYS MESSINA ITALY UNIV MESSINA,DEPT PHYS MESSINA ITALY UNIV ROME,DEPT PHYS ROME ITALY UNIV ROME,DEPT BIOL UMANA ROME ITALY UNIV BARI,IST BIOL GEN BARI ITALY UNIV ROME,DEPT MOL BIOL ROME ITALY
Titolo Testata:
Physica. B, Condensed matter
, volume: 234, anno: 1997,
pagine: 225 - 227
SICI:
0921-4526(1997)234:<225:SMOSRA>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUBUNITS;
Keywords:
SMALL-ANGLE NEUTRON SCATTERING; RIBOSOMES; SULFOLOBUS SOLFATARICUS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
7
Recensione:
Indirizzi per estratti:
Citazione:
G. Briganti et al., "SANS MEASUREMENTS ON SULFOLOBUS-SOLFATARICUS RIBOSOME AS A FUNCTION OF TEMPERATURE AND MAGNESIUM CONCENTRATION", Physica. B, Condensed matter, 234, 1997, pp. 225-227

Abstract

The ribosomes of the extremely thermophylic archaebacterium Sulfolobus solfataricus (optimal growth at T = 87 degrees C) are stable and active at temperatures close to 90 degrees C, in spite of the fact that their composition is very similar to the ribosomes of the mesophilic bacterium E. coli, growing at 37 degrees C. We present the first SANS analysis of the intact S. solfataricus 70S monomers as well as of the isolated 30S and 50S subunits as a function of the temperature and the magnesium ion concentration. Our results indicate that, under conditions similar to those employed for the analysis of E. coli ribosomes, supramolecular aggregates are present in S. solfataricus, their extent depending on temperature, ribosome concentration and magnesium ion content. Only above 70 degrees C changes in the scattering profile are observed, concomitant with the specific biological activation of this kindof ribosome.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/09/20 alle ore 09:43:42