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Titolo:
THE EFFECTS OF DONOR STAGE ON THE SURVIVAL AND FUNCTION OF EMBRYONIC STRIATAL GRAFTS IN THE ADULT-RAT BRAIN .2. CORRELATION BETWEEN POSITRON EMISSION TOMOGRAPHY AND REACHING BEHAVIOR
Autore:
FRICKER RA; TORRES EM; HUME SP; MYERS R; OPACKAJUFFREY J; ASHWORTH S; BROOKS DJ; DUNNETT SB;
Indirizzi:
UNIV CAMBRIDGE,DEPT EXPT PSYCHOL,DOWNING ST CAMBRIDGE CB2 3EB ENGLAND UNIV CAMBRIDGE,DEPT EXPT PSYCHOL CAMBRIDGE CB2 3EB ENGLAND HAMMERSMITH HOSP,MRC,CYCLOTRON UNIT,PET METHODOL GRP,CLIN SCI CTR LONDON W12 0HS ENGLAND UNIV CAMBRIDGE,MRC,CAMBRIDGE CTR BRAIN REPAIR CAMBRIDGE CB2 3EB ENGLAND
Titolo Testata:
Neuroscience
fascicolo: 3, volume: 79, anno: 1997,
pagine: 711 - 721
SICI:
0306-4522(1997)79:3<711:TEODSO>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACID-LESIONED STRIATUM; UNILATERAL NEOSTRIATAL LESIONS; ELECTRON-MICROSCOPIC ANALYSIS; SKILLED FORELIMB USE; HUNTINGTONS-DISEASE; SUBSTANTIA-NIGRA; NIGROSTRIATAL PATHWAY; MINIMAL CONNECTIVITY; PARKINSONS-DISEASE; C-11 RACLOPRIDE;
Keywords:
EMBRYONIC STRIATAL GRAFTS; POSITRON EMISSION TOMOGRAPHY; RACLOPRIDE; SCH 23390; DOPAMINE RECEPTORS; PAW REACHING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
78
Recensione:
Indirizzi per estratti:
Citazione:
R.A. Fricker et al., "THE EFFECTS OF DONOR STAGE ON THE SURVIVAL AND FUNCTION OF EMBRYONIC STRIATAL GRAFTS IN THE ADULT-RAT BRAIN .2. CORRELATION BETWEEN POSITRON EMISSION TOMOGRAPHY AND REACHING BEHAVIOR", Neuroscience, 79(3), 1997, pp. 711-721

Abstract

Grafts of embryonic striatal primordia are able to elicit behaviouralrecovery in rats which have received an excitotoxic lesion to the striatum, and it is believed that the P zones or striatal-like tissue within the transplants play a crucial role in these functional effects. We performed this study to compare the effects of different donor stageof embryonic tissue on both the morphology (see accompanying paper) and function of striatal transplants. Both the medial and lateral ganglionic eminence was dissected from rat embryos of either 10 mm, 15 mm, 19 mm, or 23 mm crown-rump length, and implanted as a cell suspension into adult rats which had received an ibotenic acid lesion 10 days prior to transplantation. After four months the animals were tested on the ''staircase task'' of skilled forelimb use. At 10-14 months rats from the groups which had received grafts from 10 mm or 15 mm donor embryos were taken for positron emission tomography scanning in a small diameter postiron emission tomography scanner, using ligands to the dopamine D-1 and D-2 receptors, [C-11]SCH 23390 and [C-11]raclopride, respectively. A lesion-alone group was also scanned with the same ligands for comparison. Animals which had received transplants from the 1O mm donors showed a significant recovery with their contralateral paw on the ''staircase test''. No other groups showed recovery on this task. Similarly, the animals with grafts from the youngest donors showed a significant increase in D-1 and D-2 receptor binding when compared to thelesion-alone group. No increase in signal was observed with either ligand in the group which had received grafts from 15 mm donors. Successin paw reaching showed a strong correlation to both the positron emission tomography signal obtained and the P zone volume of the grafts. These results suggest that striatal grafts from younger donors (1O mm CRL) give greater behavioural recovery than grafts prepared from older embryos. This recovery is due to both the increased proportion of striatal-like tissue within the grafts and an increase in functional D-1 and D-2 dopamine receptors measured by positron emission tomography, i.e. a more extensive integration of the graft with the host brain. (C) 1997 IBRO. Published by Elsevier Science Ltd.

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Documento generato il 10/07/20 alle ore 19:13:37