Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
THE EFFECTS OF DONOR STAGE ON THE SURVIVAL AND FUNCTION OF EMBRYONIC STRIATAL GRAFTS IN THE ADULT-RAT BRAIN .1. MORPHOLOGICAL-CHARACTERISTICS
Autore:
FRICKER RA; TORRES EM; DUNNETT SB;
Indirizzi:
UNIV CAMBRIDGE,DEPT EXPT PSYCHOL,DOWNING ST CAMBRIDGE CB2 3EB ENGLAND UNIV CAMBRIDGE,DEPT EXPT PSYCHOL CAMBRIDGE CB2 3EB ENGLAND UNIV CAMBRIDGE,MRC CAMBRIDGE,CTR BRAIN REPAIR CAMBRIDGE CB2 3EB ENGLAND UNIV CAMBRIDGE,MRC,CTR BRAIN REPAIR CAMBRIDGE CB2 3EB ENGLAND
Titolo Testata:
Neuroscience
fascicolo: 3, volume: 79, anno: 1997,
pagine: 695 - 710
SICI:
0306-4522(1997)79:3<695:TEODSO>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
UNILATERAL NEOSTRIATAL LESIONS; TISSUE GRAFTS; MESSENGER-RNA; CELLULAR-LOCALIZATION; HUNTINGTONS-DISEASE; NEURAL IMPLANTS; GENE-EXPRESSION; SPINY NEURONS; TRANSPLANTS; DOPAMINE;
Keywords:
EMBRYONIC STRIATAL GRAFTS; P ZONES; ACETYLCHOLINESTERASE; DARPP-32; TYROSINE HYDROXYLASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
68
Recensione:
Indirizzi per estratti:
Citazione:
R.A. Fricker et al., "THE EFFECTS OF DONOR STAGE ON THE SURVIVAL AND FUNCTION OF EMBRYONIC STRIATAL GRAFTS IN THE ADULT-RAT BRAIN .1. MORPHOLOGICAL-CHARACTERISTICS", Neuroscience, 79(3), 1997, pp. 695-710

Abstract

The effects of the stage of donor embryos on the survival of grafts from different neuronal cell types have been well documented. Indeed, this parameter has been shown to be highly important in the survival and function of transplants of various tissues of the CNS. However this question has not been addressed in grafts of embryonic striatal tissuetransplanted into animal models of Huntington's disease. In this study, rats which had received a unilateral ibotenic acid lesion in the dorsal striatum received grafts from a standard dissection of embryonic striatal primordium taken from donors of embryonic stage either E14, E16, E17 or E19 days. Three months after transplantation six rats from each group were killed for analysis of graft survival and morphology. The remaining animals in each group were killed between 10 and 14 months after grafting. Graft morphology was detected using a range of markers including: acetylcholinesterase and Cresyl Violet, the 32,000 mol.wt dopamine- and cyclic AMP-regulated phosphoprotein (DARPP-32), tyrosine hydroxylase and striatally-enriched phosphatase. All the grafts from different donor stages survived well at both time-points and Cresyl Violet staining indicated neuronal cell types spread throughout the grafts. The transplants were seen to have a characteristic ''patchy'' appearance with areas of dense AChE activity and DARPP-32 immunopositivity interspersed with areas of much lighter expression. These areas also co-localized consistently with striatally-enriched phosphatase andtyrosine hydroxylase expression, indicating that they comprised the striatal-like compartment of the graft (the so called P zones, containing cells of the mature striatum), and receiving specific afferent input from the host dopaminergic system. There was no significant difference in total graft volume, when comparing individual groups at both time-points from grafting. However, when comparing the volume of the P zones, the striatal primordium from the youngest donor stages (E14 and E16) produced grafts with a significantly higher proportion of striatal-like tissue. Therefore, in order to increase the proportion of striatal tissue within these grafts, tissue from younger embryonic donors should be used. This has important implications in the application of this model towards clinical trials in Huntington's disease. (C) 1997 IBRO. Published by Elsevier Science Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 06:19:02