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Titolo:
NICOTINIC RECEPTOR MEDIATES SPONTANEOUS GABA RELEASE IN THE RAT DORSAL MOTOR NUCLEUS OF THE VAGUS
Autore:
BERTOLINO M; KELLAR KJ; VICINI S; GILLIS RA;
Indirizzi:
GEORGETOWN UNIV,SCH MED,DEPT PHARMACOL WASHINGTON DC 20007 GEORGETOWN UNIV,SCH MED,DEPT PHYSIOL & BIOPHYS WASHINGTON DC 20007
Titolo Testata:
Neuroscience
fascicolo: 3, volume: 79, anno: 1997,
pagine: 671 - 681
SICI:
0306-4522(1997)79:3<671:NRMSGR>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL NERVOUS-SYSTEM; ACETYLCHOLINE-RECEPTORS; PATCH-CLAMP; CHOLINE-ACETYLTRANSFERASE; GASTRIC-MOTILITY; NEURONS; BRAIN; MODULATION; CURRENTS; SUBUNIT;
Keywords:
BRAIN SLICES; NEURONAL ACETYLCHOLINE RECEPTORS; PARASYMPATHETIC NUCLEUS; PATCH-CLAMP TECHNIQUE; SYNAPTIC TRANSMISSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
50
Recensione:
Indirizzi per estratti:
Citazione:
M. Bertolino et al., "NICOTINIC RECEPTOR MEDIATES SPONTANEOUS GABA RELEASE IN THE RAT DORSAL MOTOR NUCLEUS OF THE VAGUS", Neuroscience, 79(3), 1997, pp. 671-681

Abstract

Spontaneous postsynaptic currents were investigated in neurons of thecaudal portion of the dorsal motor nucleus of the vagus using the patch-clamp technique to study the effect of neuronal nicotinic acetylcholine receptor activation on synaptic transmission. In voltage-clamped neurons, bath application of nicotine (1-30 mu M) elicited a concentration-dependent increase in the frequency of the spontaneous synaptic currents. The effect was also observed with application of the nicotinic receptor agonists epibatidine (10 nM) and cytisine (10 mu M). Mecamylamine (20 mu M) and curare (50 mu M), two nicotinic receptor antagonists, both decreased the effect of 3 mu M nicotine on the frequency of the spontaneous postsynaptic currents. This effect of 3 mu M nicotine was also blocked by 20 mu M bicuculline, a competitive antagonist of the GABA(A) receptor; in contrast, it was not affected by 1 mM kynurenic acid, an antagonist of the ionotropic glutamate receptor. In the presence of 1 mu M tetrodotoxin, 3 mu M nicotine was unable to affect thesynaptic activity. Our findings suggest the existence of nicotinic receptors on GABAergic axons projecting to the vagal motoneurons. Because the effect is completely abolished by 1 mu M tetrodotoxin, the nicotinic receptors are not localized on the presynaptic nerve terminal andtheir action on the GABA release requires the propagation of an action potential from their location to the synaptic terminal. This effect of nicotinic receptor activation on spontaneous GABA release in the dorsal motor nucleus of the vagus may have an important role in the regulation of gastrointestinal motility. (C) 1997 IBRO. Published by Elsevier Science Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 22:13:29